Soluble P-selectin levels in patients with obstructive sleep apnea: a systematic review and meta-analysis

xlomafota13 shared this article with you from Inoreader Soluble P-selectin levels in patients with obstructive sleep apnea: a systematic review and meta-analysis Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2021 May 5. doi: 10.1007/s00405-021-06831-4. Online ahead of print. ABSTRACT PURPOSE: Obstructive sleep apnea (OSA) patients are at increased risk for cardiovascular disease, stroke, atherosclerosis, hypertension, and venous thromboembolism. Elevated soluble P-selectin (sP-selectin) levels are also associated with increased risk of above diseases. But whether sP-selectin levels in OSA patients are higher than their counterparts remain unclear, since previous studies yielded inconsistent results. Therefore, a meta-analysis is warranted. METHODS: PubMed, Embase, Cochrane Library, and Web of Science databases were searched for eligible studies. Studies were included if they reported sP-selectin levels of both OSA patients and non-OSA controls. Standardized mean differences (SMDs) with 95% confidence intervals (CIs) were calculated to determine the effect sizes. RESULTS: Nine eligible studies were fin ally evaluated. When all the studies were pooled, sP-selectin levels in OSA patients were significantly higher than that in controls (SMD = 0.54, 95% CI 0.29-0.78, I2 = 66%, p < 0.0001). In the subgroup analysis based on BMI matched groups, sP-selectin levels were significantly higher in OSA patients than that in controls (SMD = 0.52, 95% CI 0.27-0.76, I2 = 23%, p < 0.0001). In the subgroup analysis stratified by blood source, either serum sP-selectin levels or plasma sP-selectin levels in OSA patients were higher than that in controls. Moderate-to-severe OSA patients had significant higher sP-selectin levels (SMD = 0.80, 95% CI 0.45-1.15, I2 = 67%, p < 0.00001), while mild OSA patients showed no significant difference with controls. CONCLUSION: The pooled results reveal that OSA patients have higher sP-selectin levels than non-OSA controls. This conclusion remains unaltered in all subgroups other than the subgroup of mild OSA patients . Additional studies are warranted to better identify the role of sP-selectin as a potential biomarker in OSA patients. PMID:33950356 | DOI:10.1007/s00405-021-06831-4 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Long non-coding RNA CYTOR regulates proliferation and metastasis of colon cancer cells through regulating miRNA-105/PTEN axis

xlomafota13 shared this article with you from Inoreader Long non-coding RNA CYTOR regulates proliferation and metastasis of colon cancer cells through regulating miRNA-105/PTEN axis Via International Journal of Clinical and Experimental Pathology Int J Clin Exp Pathol. 2021 Apr 15;14(4):434-443. eCollection 2021. ABSTRACT Colon cancer is a common malignancy, and its incidence and mortality have been increasing in recent years. This study aims to explore the regulation of long non-coding RNA CYTOR on proliferation and metastasis of colon cancer cells through miRNA-105/PTEN axis. Real-time quantitative PCR (qRT-PCR) disclosed that expression of CYTOR was significantly decreased in colon cancer tissues, compared with that of adjacent normal tissues, while miRNA-105 was significantly increased. Correlation study found that CYTOR was negatively correlated with miR-105. The proliferation, migration, and invasion rates of the LoVo cells with highly expressed CYTOR were significantly slower. miR-105 mimic could suppress the decrease in proliferation, migration, and invasion rates of colon cancer cells caused by overexpression of CYTOR. Additionally, the proliferation, migration, and in vasion rates of the LoVo cells in miR-105 inhibition group were significantly slower. The Starbase database predicted the targeting of miR-105 by CYTOR, and qRT-PCR and dual luciferase reporter gene method were used to verify the targeting relationship of CYTOR and miRNA-105/PTEN axis. In conclusion, CYTOR can inhibit the proliferation and metastasis of colon cancer cells through targeted inhibition of the miR-105/PTEN axis. PMID:33936365 | PMC:PMC 8085819 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Effect of resveratrol on abnormal bone remodeling and angiogenesis of subchondral bone in osteoarthritis

xlomafota13 shared this article with you from Inoreader Effect of resveratrol on abnormal bone remodeling and angiogenesis of subchondral bone in osteoarthritis Via International Journal of Clinical and Experimental Pathology Int J Clin Exp Pathol. 2021 Apr 15;14(4):417-425. eCollection 2021. ABSTRACT PURPOSE: The effect of resveratrol on subchondral bone in osteoarthritis was explored by constructing a mouse model of osteoarthritis and giving resveratrol as intervention. METHODS: The degree of proteoglycan loss in articular cartilage was assessed by safranine fast green staining. The expressions of Lubricin and Aggrecan, COLX, and MMP-13, the co-expression of CD31 and Endomucin, and the expression of angiogenesis-related factors were determined by immunohistochemistry. TRAP stain and immunostaining were used to assess abnormal subchondral bone resorption and bone formation. Angiography was employed to analyze the effect of resveratrol on the proliferation of subchondral bone vessels. RESULTS: Resveratrol inhibited cartilage thickening and the increase of COLX and MMP-13 expression, delayed the loss of proteoglycan, Lubricin, and Aggrecan, and i nhibited osteoclast differentiation by up-regulating osteoprotegerin (OPG) and down-regulating the expression of RANKL. Angiography showed that resveratrol can reduce the abnormally elevated number and volume of blood vessels in the subchondral bone. Immunostaining showed that resveratrol inhibited CD31hiEmcnhi angiogenesis and high expression of VEGFA and Angiopoietin-1. CONCLUSION: Resveratrol inhibits osteoclast differentiation and reduces active bone resorption by regulating the OPG/RANKL/RANK pathway, and inhibits the abnormal proliferation of CD31hiEmcnhi blood vessels by downregulating the expression of VEGFA and Angiopoiein-1, thereby eliminating the pathologic coupling mechanism of osteogenesis and vascularization, and delaying the progression of osteoarthritis. PMID:33936363 | PMC:PMC8085829 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Accuracy, predictability and prognostic implications of fine needle aspiration biopsy for parotid gland tumors: A retrospective case series

xlomafota13 shared this article with you from Inoreader Accuracy, predictability and prognostic implications of fine needle aspiration biopsy for parotid gland tumors: A retrospective case series Via Clinical Otolaryngology Abstract Objective To evaluate the precision and utility of Fine‐needle aspiration (FNA) in differentiating between benign and malignant parotid tumors, and the implications of FNA results on management and outcomes. Design Retrospective case series. Setting Tertiary medical center. Participants All adults who underwent preoperative FNA, followed by postoperative histological examination, between 1986‐2014. Main outcome measures Differences in clinical management and outcomes of patients with parotid masses in light of FNA results. Results We analyzed 505 samples from 485 patients. According to histopathological results, preoperative FNA successfully identified benign tumors in 89% of the cases (362/405), and only 59% of malignant tumors (59/100). Overall sensitivity and specificity of FNA in distinguishing between different subtypes of benign lesions were 80% and 99%, respectively, whereas positive predictive value (PPV) and negative predictive value (NPV) were 85% and 98%. Moreover, malignant lesions subtyping had high false‐positive and false‐negative rates with sensitivity, specificity, PPV and NPV of 44%, 100%, 75% and 99%, respectively. Additionally, when FNA falsely classified malignant tumors as benign, surgeries were inappropriately delayed and the durations of surgeries and hospitalizations were shorter, compared to true malignant‐FNA results. Interestingly, survival was not affected in falsely benign lesions that were mostly low‐grade, conversely non‐diagnostic FNA for malignant tumors resulted in decreased survival. Conclusions Our findings highlight the limitations of FNA as a decision‐making tool in preoperative evaluation of parotid masses. Clinicians should take into account that FNA is inaccurate for identifying specific subtypes of malignant lesions, which may eventually delay treatment and influence outcome. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Spinal drainage complications after aortic surgery

xlomafota13 shared this article with you from Inoreader Spinal drainage complications after aortic surgery Via leak of cerebrospinal fluid J Vasc Surg. 2021 Apr 30:S0741-5214(21)00671-6. doi: 10.1016/j.jvs.2021.04.031. Online ahead of print. ABSTRACT INTRODUCTION: Spinal drain (SD) placement is an adjunct used in open and endovascular aortic surgery to mitigate the risk of spinal cord injury. SD placement can lead to subdural hematoma and intracranial hemorrhage (SDH/ICH). Previous studies have highlighted a correlation between incidence of SDH/ICH and amount of cerebrospinal fluid (CSF) drained. We have two philosophies of SD management in our institution. One protocol allows fluid removal for pressure > 10 cm H2O with no volume restriction. A second, similar protocol restricts CSF drainage to < 25 ml/hr. We examined SD complications and the influence of volume restriction. METHODS: Patients were identified according to the Current Procedure Terminology codes (CPT) for SD placement, thoracic endovascular aortic repair, fenestrated/branched endovascular aortic repair , endovascular abdominal aortic repair, open thoracic or thoracoabdominal aortic repair between January 1, 2012 and December 31, 2015. Patients' demographics included age, gender, race, body mass index and comorbidities such as hypertension, chronic obstructive pulmonary disease, stroke, transient ischemic attack, diabetes mellitus, bleeding disorder and connective tissue disorders. Management protocol was divided as volume independent (VI) or volume dependent (VD) by physician order. Postoperative complications related to the SD were noted. RESULTS: We identified 948 patients who had a SD placed during the study period, 473 were done prior to aortic surgeries. 364 patients (77%) underwent endovascular aortic surgery. Mean age at the time of procedure was 67.2 years and 66% of patients were male. 39 patients (8.3%) were noted to have connective tissue disorders. Bloody SD placement occurred in 14 patients (3.1%) requiring rescheduling of the operation. SDH/ICH occurred in 11 p atients (2.3%), post-operative blood tinged SD output in 94 patients (19.9 %) and 22 patients (4.7 %) had a CSF leak after SD removal. The incidence of SDH/ICH was not affected by the management protocol (2.6% VI Vs 2.0% VD, p=0.66), while the incidence of postoperative blood tinged SD output was significantly higher in the VI group (25.1% VI vs 15.0% VD, p=0.006). Perioperative low dose aspirin (81 mg) and prophylactic subcutaneous heparin did not increase the incidence of SDH/ICH. Post-operative thrombocytopenia was found to be associated with higher incidence of SDH/ICH (median 86,000 Vs 113,000, p=0.002). CONCLUSION: Severe complications of SD placement (SDH/ICH) occurs in 2.3% of SD patients undergoing aortic surgery and the risk is higher in the setting of post-operative thrombocytopenia. SD volume limitation, blood tinged SA drainage, antiplatelet medication and low dose heparin do not affect the risk of SDH/ICH. The risks of spinal drains for aortic surgery should be b alanced against potential benefits. PMID:33940078 | DOI:10.1016/j.jvs.2021.04.031 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Transorbital neuroendoscopic surgery as a mainstream neurosurgical corridor: a systematic review

xlomafota13 shared this article with you from Inoreader Transorbital neuroendoscopic surgery as a mainstream neurosurgical corridor: a systematic review Via leak of cerebrospinal fluid World Neurosurg. 2021 Apr 30:S1878-8750(21)00647-1. doi: 10.1016/j.wneu.2021.04.104. Online ahead of print. ABSTRACT BACKGROUND: Transorbital neuroendoscopic surgery (TONES) offers a new level of minimally invasive, minimally disfiguring skull base surgery with maximal surgical visualization. METHODS: This review systematically assesses the body of published anatomical (cadaveric) and clinical evidence for the approach. PubMed, Cochrane Library, Ovid MEDLINE, and EMBASE were systematically searched for publications where the TONES surgical technique was used in an anatomical, clinical, or combined study. The outcomes of interest included identification of the pathologies, operative outcomes, and complication rates. RESULTS: Twenty-three papers were selected for this systematic review: 10 were purely anatomical, 10 were clinical, and 3 had both clinical and cadaveric components. The papers reported 69 patients undergoing transorbit al or combined transorbital and transnasal intervention. A total of 30 cases of cerebrospinal fluid leak were documented; of these, 28 (93%) had successful resolution, 2 (7%) had recurrence, and 5 (15%) experienced complications. A total of 31 tumors were biopsied (n=1), resected (n=22), or debulked (n=8). Meningiomas were the most common lesion managed via TONES, with 5 of 7 patients with meningioma who reported preoperative neurological deficits experiencing an improvement in extraocular movement impairment, visual acuity, proptosis, and ptosis. Transient postoperative clinical sequelae, including diplopia and ptosis, were increasingly associated with the superior lid crease incision and the sole transorbital approach. CONCLUSIONS: TONES is a significant development in transorbital skull base surgery. However, comprehensive, robust, comparative analyses and increasing use and generalizability of this technique in skull base surgery are awaited. PMID:33940270 | DOI:10.1016/j.wneu.2021.04.104 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Supracricoid partial laryngectomy with cricohyoidoepiglottopexy for patients with laryngeal cicatricial stenosis: Safety and efficacy

xlomafota13 shared this article with you from Inoreader Supracricoid partial laryngectomy with cricohyoidoepiglottopexy for patients with laryngeal cicatricial stenosis: Safety and efficacy Via Head & Neck Abstract Background We assessed the safety and efficacy of supracricoid partial laryngectomy with cricohyoidoepiglottopexy (SCL‐CHEP) in patients with laryngeal cicatricial stenosis. Methods Sixteen patients receiving SCL‐CHEP for severe laryngeal cicatricial stenosis between 2017 and 2018 were reviewed. Decannulation rate and tracheostomy closure time were used to evaluate efficacy. The Voice Handicap Index‐10 (VHI‐10), Voice‐related Quality of Life (V‐RQOL) scale and Grade, Roughness, Breathiness, Asthenia, and Strain (GRBAS) scale were used to assess vocal function. Fiberoptic endoscopic evaluation of swallowing (FEES) was performed and the Penetration‐Aspiration Scale (PAS), Eating Assessment Tool‐10 (EAT‐10), and Swallow Quality of Life Questionnaire (SWAL‐QOL) were used to assess swallowing function. Results Thirteen patients (81.25%) were decannulated successfully. The average tracheostomy closure time was 45.15 days. There was no observed postoperative complications or recurrence of stenosis. VHI‐10 and V‐RQOL scores showed significantly improved V‐RQOL (p < 0.05). FEES‐PAS, EAT‐10, and SWAL‐QOL showed no swallowing function damage. Conclusions SCL‐CHEP is effective and safe for patients with severe laryngeal cicatricial stenosis. Accurate pre‐procedure evaluation is especially important for patient selection and surgical success. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.