Reconstruction of Plasmodium vivax outbreaks in a low malaria endemic setting utilizing conventional restriction fragment length polymorphism

xlomafota13 shared this article with you from Inoreader Reconstruction of <em>Plasmodium vivax</em> outbreaks in a low malaria endemic setting utilizing conventional restriction fragment length polymorphism Via International Journal of Molecular Epidemiology and Genetics Int J Mol Epidemiol Genet. 2021 Feb 15;12(1):9-15. eCollection 2021. ABSTRACT Suriname is on track to eliminate local malaria transmission. P. vivax malaria reemerged in March and September 2019 in the Amerindian village Palumeu, free of malaria for two years and concurrently, a case was reported in another village Alalaparoe. The outbreaks were contained through targeted interventions including Mass Drug Administration (MDA). Molecular outbreak analysis was performed on 23 dried blood spots (DBS) using combined polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP) with Pvmsp-1 F2 and Pvmsp-3α as polymorphic marker genes. Independent controls substantiated the discriminating capacities of the utilized PCR-RFLP method. All isolates from the first and second Palumeu outbreak shared a distinctive haplotype presuming single clonal lineage. An imported case probably triggered the first outbreak, while a delayed episode, prompted by withdrawal of drug pressure at the end of the prophylactic MDA, was suggested as source of the second outbreak. A diverging variant was demonstrated in Alalaparoe, implicating an infection from a different source. PCR-RFLP proved to be a useful molecular tool for P. vivax outbreak management in low endemic malaria settings. PMID:33859783 | PMC:PMC8044709 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.