Abstract
Aim
Electroacupuncture (EA) regulates distant body physiology through somatic sensory autonomic reflexes, balances the microbiome and can promote the release of immune cells into bloodstream, thereby inhibits severe systemic inflammation. This makes it possible to use EA as an integrated treatment for periodontitis.
Materials and methods
In this study, EA was applied to the ST36 acupoints in a ligature-induced periodontitis (LIP) mouse model. Then the effects of EA on periodontal myeloid cells, cytokines and microbiome were comprehensively analyzed using flow cytometry, qPCR and 16S sequencing.
Results
Results demonstrated that EA could significantly relieve periodontal bone resorption. EA also suppressed infiltration of macrophages and neutrophils, reduced gene expression of the pro-inflammatory cytokines IL-1β, IL-6, IL-17 and TNF-α, while increased expression of anti-inflammatory factors IL-4 and IL-10 in periodontal tissues. Moreover, composition of the periodontal microbiome was regulated by EA, finding that complex of microbiota, including supragingival Veillonella, subgingival Streptococcus, and subgingival Erysipelatoclostridium, were significantly reduced. Meanwhile, nitrate and nitrate-related activities of subgingival microbiota were reversed. Network analysis revealed close relationships among Veillonella, Streptococcus, and Bacteroides.
Conclusion
Our study indicated that EA effectively alleviated inflammation and bone resorption in LIP mice, potentially via regulation of myeloid cells, cytokines, and periodontal microbiome.
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