Cancers, Vol. 12, Pages 928: Cytotoxic Effect of Trabectedin In Human Adrenocortical Carcinoma Cell Lines and Primary Cells
Cancers doi: 10.3390/cancers12040928
Authors:
Andrea Abate
Elisa Rossini
Sara Anna Bonini
Martina Fragni
Deborah Cosentini
Guido Albero Massimo Tiberio
Diego Benetti
Constanze Hantel
Marta Laganà
Salvatore Grisanti
Massimo Terzolo
Maurizio Memo
Alfredo Berruti
Sandra Sigala
Mitotane is the only drug approved for the treatment of adrenocortical carcinoma (ACC). The regimen to be added to mitotane is a chemotherapy including etoposide, doxorubicin, and cisplatin. This pharmacological approach, however, has a limited efficacy and significant toxicity. Evidence indicates that ACC seems to be sensitive to alkylating agents. Trabectedin is an anti-tumor drug that acts as an alkylating agent with a complex mechanism of action. Here, we investigated whether trabectedin could exert a cytotoxic activity in in vitro cell models of ACC. Cell viability was evaluated by MTT assay on ACC cell lines and primary cell cultures. The gene expression was evaluated by q-RT-PCR, while protein expression and localization were studied by Western blot and immunocytochemistry. Combination experiments were performed to evaluate their interaction on ACC cell line viability. Trabectedin demonstrated high cytotoxicity at sub-nanomolar concentrations in ACC cell lines and patient-derived primary cell cultures. The drug was able to reduce /β catenin nuclear localization, although it is unclear whether this effect is involved in the observed cytotoxicity. Trabectedin/mitotane combination exerted a synergic cytotoxic effect in NCI-H295R cells. Trabectedin has antineoplastic activity in ACC cells. The synergistic cytotoxic activity of trabectedin with mitotane provides the rationale for testing this combination in a clinical study.
Great Dr. imoloa herbal medicine is the perfect cure for the HIV virus, I was diagnosed of HIV for 8 years, and every day I always looking for research to find the perfect way to get rid of this terrible disease because I always know that what we need because our health is on earth . So, on my internet search I saw several testimonies about how Dr. imoloa can cure HIV with powerful herbal medicines. I decided to contact this man, I contacted him for herbal medicines that I received via DHL courier service. And he guided me how. I asked him for a solution to take herbal medicine for two weeks. And then he instructed me to go check what I was doing. Look at me (HIV NEGATIVE). Thank God for Dr. Imoloa for using a powerful herbal remedy to cure me. he also has a cure for diseases such as Parkison's disease, vaginal cancer, epilepsy, anxiety disorders, autoimmune diseases, back pain, sprains, bipolar disorder, brain tumors, malignant, bruxism, bulimia, cervical disc disease, cardiovascular disease, cardiovascular disease, sprains, bipolar disorder, brain tumors, malignant, bruxism, bulimia, cervical disc disease, cardiovascular disease, cardiovascular disease, sprains, bipolar disorder, brain tumors, malignant, bruxism, bulimia, cervical disc disease, cardiovascular disease , chronic respiratory diseases, mental and behavioral disorders, Cystic Fibrosis, Hypertension, Diabetes, asthma, autoimmune mediated arthritis. Chronic kidney disease, arthritis, back pain, impotence, feta alcohol spectrum, Dysthymic Disorders, Eczema, skin cancer, tuberculosis, Chronic Fatigue Syndrome, constipation, inflammatory bowel disease, bone cancer, lung cancer, mouth sores, oral cancer, body pain, fever, hepatitis ABC, syphilis, diarrhea, Huntington's disease, back acne, chronic kidney failure, Addison's disease, Chronic Disease, Crohn's Disease, Cystic Fibrosis, Fibromyalgia, Inflammatory Bowel Disease, Fungal Nail Disease, Paralysis, Celia Disease , Lymphoma, Major Depression, Malignant Melanoma, Mania, Melorheostosis, Meniere's Disease, Mucopolysaccharidosis, Multiple Sclerosis, Muscle Dystrophy, Rheumatoid Arthritis, Alzheimer's Disease email- drimolaherbalmademedicine@gmail.com / or {whatssapp..+2347081986098} website- www.drimolaherbalmademedicine.wordpress.com
ΑπάντησηΔιαγραφή