Food workers as a reservoir of extended-spectrum cephalosporin-resistant Salmonella in Japan.

Food workers as a reservoir of extended-spectrum cephalosporin-resistant Salmonella in Japan.:

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Food workers as a reservoir of extended-spectrum cephalosporin-resistant Salmonella in Japan.

Appl Environ Microbiol. 2020 Apr 10;:

Authors: Shigemura H, Sakatsume E, Sekizuka T, Yokoyama H, Hamada K, Etoh Y, Carle Y, Mizumoto S, Hirai S, Matsui M, Kimura H, Suzuki M, Onozuka D, Kuroda M, Inoshima Y, Murakami K

Abstract

Dissemination of extend-spectrum cephalosporin (ESC)-resistant Salmonella, especially extended-spectrum β-lactamase (ESBL)-producing Salmonella, is a concern worldwide. Here, we assessed Salmonella carriage by food workers in Japan to clarify the prevalence of ESC-resistant Salmonella harboring bla CTX-M We then characterized the genetic features, such as transposable elements, of bla CTX-M-harboring plasmids using whole-genome sequencing. A total of 145,220 stool samples were collected from food workers, including cooks and servers from several restaurants as well as food factory workers, from January-October 2017. Isolated salmonellae were subjected to antimicrobial susceptibility testing (disk diffusion method), and whole-genome sequencing was performed for Salmonella strains harboring bla CTX-M Overall, 164 Salmonella isolates (0.113%) were recovered from 164 samples, from which we estimated that at least 0.113% (95% confidence interval (CI): 0.096%-0.132%) of food workers may carry Salmonella Based on this estimation, 3,473 (95% CI: 2,962-4,047) individuals among the 3,075,330 Japanese food workers are likely to carry Salmonella Of the 158 culturable isolates, seven showed resistance to ESCs: three isolates harbored bla CMY-2 and produced AmpC β-lactamase, while four ESBL-producing isolates harbored bla CTX-M-14 (n = 1, Salmonella enterica serovar Senftenberg) or bla CTX-M-15 (n = 3, S enterica serovar Haardt). bla CTX-M-15 was chromosomally-located in the S Haardt isolates, which also contained ISEcp1, while the S. Senftenberg isolate contained an IncFIA(HI1)/IncHI1A/IncHI1B(R27) hybrid plasmid carrying bla CTX-M-14 along with ISEcp1 This study indicates that food workers may be a reservoir of ESBL-producing Salmonella and associated genes. Thus, these workers may contribute to the spread of bla CTX-M via plasmids or mobile genetic elements such as ISEcp1 IMPORTANCE Antimicrobial-resistant Salmonella arise in farm environments through imprudent use of antimicrobials. Subsequently, these antimicrobial-resistant strains, such as extended-spectrum β-lactamase (ESBL)-producing Salmonella, may be transmitted to humans via food animal-derived products. Here, we examined Salmonella carriage among food handlers in Japan. Overall, 164 out of 145,220 fecal samples (0.113%) were positive for Salmonella Among the 158 tested isolates, four were identified as ESBL-producing isolates carrying ESBL determinants bla CTX-M-15 or bla CTX-M-14 In all cases, the genes co-existed with ISEcp1, regardless of whether they were located on the chromosome or on a plasmid. Our findings suggest that food workers may be a reservoir of ESBL-producing strains, and could contribute to the spread of resistance genes from farm-derived Salmonella to other bacterial species present in the human gut.

PMID: 32276982 [PubMed - as supplied by publisher]