| Related Articles |
Int J Infect Dis. 2020 Apr 03;:
Authors: Azevedo Alves Leitão G, Ignácio Olivares Olivares A, Cardoso Pimenta Y, Fernandes Delgado I, Pereira Miagostovich M, Gagliardi Leite JP, de Moraes MTB
Abstract
OBJECTIVES: This study aimed to verify the frequency, genotypes and etiological role of Human Bocavirus (HBoV) in younger Amazonian children with either acute gastroenteritis (AGE) or respiratory infections (ARI). The influence of Rotarix™ vaccination and co-infection status was investigated.
DESIGN: HBoV quantitative polymerase chain reaction (qPCR) testing of both fecal and saliva (1468 samples) from 734 children <5 months old living in the Amazon (Brazil, Guyana and Venezuela). High and median HBoV viral load samples were used for extraction, nested PCR amplification and sequencing for genotyping. HBoV mRNA detection was done by reverse transcription following DNA amplification.
RESULTS: The overall HBoV frequencies were 14.2% (69/485; AGE) and 14.1% (35/249; ARI) (p = 0.83). HBoV exclusively infected 4.5% (22/485; AGE) and 4% (10/249) of the Amazonian children (Odds ratios 1.13, 95% confidence interval= 2.42-0.52). HBoV 1 was mainly detected in feces and saliva from AGE children; and HBoV2, from ARI children. HBoV mRNA was detected only in feces. The Rotarix™ vaccination status did not affect the HBoV frequencies.
CONCLUSIONS: We suggested that, after entry into the air/oral pathways, HBoV1 continues infecting towards the intestinal tract causing AGE. HBoV2 can be a causative agent of AGE and ARI in younger Amazonian children.
PMID: 32251804 [PubMed - as supplied by publisher]
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου