Neuroglobin in Purkinje Neurons in the Rate Cerebellum in Cholestasis

Neuroglobin in Purkinje Neurons in the Rate Cerebellum in Cholestasis:

Objective. To assess the neuroglobin content of cerebellar Purkinje cells during experimental cholestasis in rats. Materials and methods. Studies were performed on paraffin sections of the cerebellar cortex processed for immunohistochemical detection of neuroglobin in Purkinje neurons in 60 mongrel male rats weighing 200–250 g after experimental treatment. Experimental animals underwent ligation of the common bile duct, while control animals underwent sham operations not preventing physiological bile outflow into the duodenum. Results. After ligation of the common bile duct, the neuroglobin content in the perikarya of cerebellar Purkinje neurons showed wavelike changes: it initially decreased on experimental days 2–20 (with a minimum on days 5–10) in conditions of cholestasis and then increased on days 45–90 of natural recovery from cholestasis (with a maximum on day 45). Conclusions. Ligation of the common bile duct produced wavelike changes in the neuroglobin content in the perikarya of cerebellar Purkinje neurons, with a significant decrease in cholestasis and an increase on spontaneous recovery from cholestasis.