Proteomic analysis of CHIKV-infected human fibroblast-like synoviocytes: identification of host factors potentially associated with CHIKV replication and cellular pathogenesis.

Proteomic analysis of CHIKV-infected human fibroblast-like synoviocytes: identification of host factors potentially associated with CHIKV replication and cellular pathogenesis.:

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Proteomic analysis of CHIKV-infected human fibroblast-like synoviocytes: identification of host factors potentially associated with CHIKV replication and cellular pathogenesis.

Microbiol Immunol. 2020 Apr 04;:

Authors: Sukkaew A, Suksatu A, Roytrakul S, Smith DR, Ubol S

Abstract

Chikungunya virus (CHIKV) is a mosquito-borne virus that causes arthralgic fever. Fibroblast-like synoviocytes play a key role in joint damage in inflammatory arthritides and can additionally serve as target cells for CHIKV infection. To get a better understanding of CHIKV-induced arthralgia, we investigated the interaction between CHIKV and synoviocytes at the protein level. A gel-enhanced liquid chromatography-mass spectrometry (GeLC-MS/MS) approach was used to examine protein expression from primary human fibroblast-like synoviocytes (HFLS) infected with clinical isolates of CHIKV at 12 and 24 hours post infection. Our analysis identified 259 and 241 proteins of known function that were differentially expressed (>1.5 or <-1.5 fold change) following CHIKV infection at 12 and 24 hpi, respectively. These proteins are involved in cellular homeostasis, including cellular trafficking, cytoskeletal organization, immune response, metabolic process, and protein modification. Some of these proteins have previously been reported to participate in arthralgia/arthritis and death of infected cells. Our results provide information on CHIKV-induced modulation of cellular proteins of HFLS at an early stage of infection, as well as highlighting biological processes associate with CHIKV infection in the main target cells of the joint. This article is protected by copyright. All rights reserved.

PMID: 32246487 [PubMed - as supplied by publisher]