Related ArticlesPrimary cilium and brain aging: role in neural stem cells, neurodegenerative diseases and glioblastoma. Ageing Res Rev. 2019 07;52:53-63 Authors: Álvarez-Satta M, Moreno-Cugnon L, Matheu A Abstract Brain aging is characterized by a progressive loss of tissue integrity and function as a consequence of impaired homeostasis and regeneration capacities. The primary cilium is a highly conserved organelle that projects from the cell surface...
Tue Mar 10, 2020 12:43
Related ArticlesMeasurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells. J Vis Exp. 2020 Feb 22;(156): Authors: Chava S, Bugide S, Gupta R, Wajapeyee N Abstract Natural killer (NK) cells are a subset of the cytotoxic lymphocyte population of the innate immune system and participate as a first line of defense by clearing pathogen-infected, malignant, and stressed cells. The ability of NK cells to...
Tue Mar 10, 2020 14:41
Related ArticlesCharacterizing Lewis Pairs Using Titration Coupled with In Situ Infrared Spectroscopy. J Vis Exp. 2020 Feb 20;(156): Authors: Hanson CS, Devery JJ Abstract Lewis acid-activation of carbonyl-containing substrates is a fundamental basis for facilitating transformations in organic chemistry. Historically, characterization of these interactions has been limited to models equivalent to stoichiometric reactions. Here, we report a method...
Tue Mar 10, 2020 14:41
Related ArticlesUniversal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids. J Vis Exp. 2020 Feb 18;(156): Authors: Gaebler AM, Hennig A, Buczolich K, Weitz J, Welsch T, Stange DE, Pape K Abstract Electroporation is a common method for transfection with different kinds of molecules by electrical permeabilization of the plasma membrane. With the increasing use of organoids as a culturing method for primary...
Tue Mar 10, 2020 14:41
Related ArticlesMicrowaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals - Using Unconjugated Primary Antibodies from the Same Host Species. J Vis Exp. 2020 Feb 21;(156): Authors: Lyu Q, Zheng HS, Laprocina K, Huang CJ Abstract Immunostaining is widely used in biomedical research to show the cellular expression pattern of a given protein. Multiplex immunostaining allows labeling using multiple primary antibodies. To minimize...
Tue Mar 10, 2020 14:41
Related ArticlesNovel Percutaneous Approach for Deployment of 3D Printed Coronary Stenosis Implants in Swine Models of Ischemic Heart Disease. J Vis Exp. 2020 Feb 18;(156): Authors: Hollowed JJ, Colbert CM, Currier JW, Nguyen KL Abstract Minimally invasive methods for creating models of focal coronary narrowing in large animals are challenging. Rapid prototyping using three-dimensionally (3D) printed coronary implants can be employed to percutaneously...
Tue Mar 10, 2020 14:41
Related ArticlesMeasuring and Manipulating Functionally Specific Neural Pathways in the Human Motor System with Transcranial Magnetic Stimulation. J Vis Exp. 2020 Feb 23;(156): Authors: Goldenkoff ER, Mashni A, Michon KJ, Lavis H, Vesia M Abstract Understanding interactions between brain areas is important for the study of goal-directed behavior. Functional neuroimaging of brain connectivity has provided important insights into fundamental processes...
Tue Mar 10, 2020 14:41
Related ArticlesMeasuring the Motor Aspect of Cancer-Related Fatigue using a Handheld Dynamometer. J Vis Exp. 2020 Feb 20;(156): Authors: Feng LR, Regan J, Shrader J, Liwang J, Alshawi S, Joseph J, Ross A, Saligan L Abstract Cancer-related fatigue (CRF) is commonly reported by patients both during and after receiving treatment for cancer. Current CRF diagnoses rely on self-report questionnaires which are subject to report and recall biases. Objective...
Tue Mar 10, 2020 14:41
Related ArticlesFluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis. J Vis Exp. 2020 Feb 18;(156): Authors: Ablondi EF, Paudel S, Sehdev M, Marken JP, Halleran AD, Rahman A, Kemper P, Saha MS Abstract Spontaneous intracellular calcium activity can be observed in a variety of cell types and is proposed to play critical roles in a variety of physiological processes. In particular,...
Tue Mar 10, 2020 14:41
Related ArticlesMuscle Velocity Recovery Cycles to Examine Muscle Membrane Properties. J Vis Exp. 2020 Feb 19;(156): Authors: Witt A, Bostock H, Z'Graggen WJ, Tan SV, Kristensen AG, Kristensen RS, Larsen LH, Zeppelin Z, Tankisi H Abstract Although conventional nerve conduction studies (NCS) and electromyography (EMG) are suitable for the diagnosis of neuromuscular disorders, they provide limited information about muscle fiber membrane properties...
Tue Mar 10, 2020 14:41
Related ArticlesUsing Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals. J Vis Exp. 2020 Feb 20;(156): Authors: Hooper SE, Eshelman AN, Cowan AN, Roistacher A, Paneitz TS, Amelon SK Abstract Body condition scoring systems and body condition indices are common techniques used for assessing the health status or fitness of a species. Body condition scoring systems are evaluator dependent...
Tue Mar 10, 2020 14:41
Related ArticlesIn Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice. J Vis Exp. 2020 Feb 20;(156): Authors: Aoki JI, Hong A, Zampieri RA, Floeter-Winter LM, Laranjeira-Silva MF Abstract Leishmania spp. are protozoan parasites that cause leishmaniases, diseases that present a wide spectrum of clinical manifestations from cutaneous to visceral lesions. Currently, 12 million people are estimated to be infected with Leishmania...
Tue Mar 10, 2020 14:41
Related ArticlesCulture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate. J Vis Exp. 2020 Feb 22;(156): Authors: Al Ahmar R, Kirby BD, Yu HD Abstract Pseudomonas aeruginosa, an opportunistic Gram-negative bacterial pathogen, can overproduce an exopolysaccharide alginate resulting in a unique phenotype called mucoidy. Alginate is linked to chronic lung infections resulting in poor prognosis in patients with cystic...
Tue Mar 10, 2020 14:41
Related ArticlesSample Preparation for Probe Electrospray Ionization Mass Spectrometry. J Vis Exp. 2020 Feb 19;(156): Authors: Takeda S, Yoshimura K, Tanihata H Abstract Mass spectrometry (MS) is a powerful tool in analytical chemistry because it provides very accurate information about molecules, such as mass-to-charge ratios (m/z), which are useful to deduce molecular weights and structures. While it is essentially a destructive analytical method,...
Tue Mar 10, 2020 14:41
Related ArticlesStepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays. J Vis Exp. 2020 Feb 21;(156): Authors: Stolwijk JA, Mildner AK, Kade C, Skiba M, Bernhardt G, Buschauer A, Huebner H, Gmeiner P, Wegener J Abstract Label-free impedance-based assays are increasingly used to non-invasively study ligand-induced GPCR activation in cell culture experiments. The approach provides real-time cell monitoring with...
Tue Mar 10, 2020 14:41
Related ArticlesA Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection. J Vis Exp. 2020 Feb 20;(156): Authors: de Vries CR, Sweere JM, Ishak H, Sunkari V, Bach MS, Liu D, Manasherob R, Bollyky PL Abstract Pseudomonas aeruginosa (P. aeruginosa) is a major nosocomial pathogen of increasing relevance to human health and disease, particularly in the setting of chronic wound infections in diabetic and hospitalized patients. There...
Tue Mar 10, 2020 14:41
Related ArticlesMeasurement of Pulse Propagation Velocity, Distensibility and Strain in an Abdominal Aortic Aneurysm Mouse Model. J Vis Exp. 2020 Feb 23;(156): Authors: Sharma N, Sun Z, Hill MA, Hans CP Abstract An abdominal aortic aneurysm (AAA) is defined as a localized dilation of the abdominal aorta that exceeds the maximal intraluminal diameter (MILD) by 1.5 times of its original size. Clinical and experimental studies have shown that small...
Tue Mar 10, 2020 14:41
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay.
Related ArticlesElectrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay. J Vis Exp. 2020 Feb 23;(156): Authors: Dimov N, McDonnell MB, Munro I, McCluskey DK, Johnston ID, Tan CKL, Coudron L Abstract Electrowetting is the effect by which the contact angle of a droplet exposed to a surface charge is modified. Electrowetting-on-dielectric (EWOD) exploits the dielectric properties of thin insulator films to...
Tue Mar 10, 2020 14:41
Related ArticlesA Standardized Protocol for Preference Testing to Assess Fish Welfare. J Vis Exp. 2020 Feb 22;(156): Authors: DePasquale C, Sturgill J, Braithwaite VA Abstract Animal welfare assessment techniques try to take into consideration the specific needs and wants of the animal in question. Providing enrichment (the addition of physical objects or conspecifics in the housing environment) is often a way to give captive animals the opportunity...
Tue Mar 10, 2020 14:41
Related ArticlesTargeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes. J Vis Exp. 2020 Feb 18;(156): Authors: Linnerz T, Kanamala M, Astin JW, Dalbeth N, Wu Z, Hall CJ Abstract Zebrafish (Danio rerio) larvae have developed into a popular model to investigate host-pathogen interactions and the contribution of innate immune cells to inflammatory disease due to their functionally conserved innate immune system. They are...
Tue Mar 10, 2020 14:41
Related ArticlesDetermining the Functional Status of the Corticospinal Tract Within One Week of Stroke. J Vis Exp. 2020 Feb 22;(156): Authors: Smith MC, Ackerley SJ, Monigatti EJ, Scrivener BJ, Stinear CM Abstract High interindividual variability in the recovery of upper limb (UL) function after stroke means it is difficult to predict an individual's potential for recovery based on clinical assessments alone. The functional integrity of the corticospinal...
Tue Mar 10, 2020 14:41
Related ArticlesAssessment of Midline Lingual Point-Pressure Somatosensation Using Von Frey Hair Monofilaments. J Vis Exp. 2020 Feb 21;(156): Authors: Etter NM, Breen SP, Alcala MIM, Ziegler GR, Hayes JE Abstract Detection and discrimination threshold estimates for oral point pressure are assessed using Von Frey Hair monofilaments. Consistent with previously published protocols, threshold estimates are determined using a two-interval forced choice...
Tue Mar 10, 2020 14:41
Related ArticlesDetermination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR. J Vis Exp. 2020 Feb 19;(156): Authors: Pradhan SK, Guzman J, Dargitz C, Switalski S, Landon M, Olek S, Samans B, Hoffmueler U, Lakshmipathy U Abstract Immune cell subtype population frequencies can have a large effect on the efficacy of T cell therapies. Current methods, like flow cytometry, have specific sample requirements, high sample input,...
Tue Mar 10, 2020 14:41
Related ArticlesOrganic Structure-directing Agent-free Synthesis for *BEA-type Zeolite Membrane. J Vis Exp. 2020 Feb 22;(156): Authors: Sakai M, Yasuda N, Tsuzuki Y, Matsukata M Abstract Membrane separation has drawn attention as a novel-energy saving separation process. Zeolite membranes have great potential for hydrocarbon separation in petroleum and petrochemical fields because of their high thermal, chemical, and mechanical strength. A *BEA-type...
Tue Mar 10, 2020 14:41
Related ArticlesAssessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface. J Vis Exp. 2020 Feb 23;(156): Authors: Tsoutsoulopoulos A, Gohlsch K, Möhle N, Breit A, Hoffmann S, Krischenowski O, Mückter H, Gudermann T, Thiermann H, Aufderheide M, Steinritz D Abstract Here, we present a specially designed modular in vitro exposure system that enables the homogenous exposure of...
Tue Mar 10, 2020 14:41
The author name Maria Maria Côrtes Thomé Lima was incorrectly captured in the original article. The correct author name should be Andrezza Maria Côrtes Thomé Lima. The original article has been corrected.
Tue Mar 10, 2020 12:38
Abstract Exposure of biological tissue to photobiomodulation therapy (PBMT) seems to increase the oxygen availability and mitochondrial electrochemical activity. With the advancement of new technologies, such as near-infrared spectroscopy (NIRS), information can be obtained about the balance between oxygen utilization and delivery by assessing local oxy- ([O2Hb]) and deoxy-myohemoglobin ([HHb]) concentrations, both measured in micromolars (μM). Consequently, NIRS can be used...
Tue Mar 10, 2020 12:38
Photobiomodulation therapy compensate the impairments of diabetic bone marrow mesenchymal stem cells
Abstract Pathophysiologic conditions associated with diabetes mellitus affect mesenchymal stem cells (MSCs), and this phenomenon may lead to some diabetic secondary complications. The present study was conducted to evaluate the impact of photobiomodulation (PBM) on rat diabetic MSC (DMSC) behavior in vitro. For the purpose of PBM, we used helium-neon laser with a wavelength of 632.8 nm at three different energy densities (0.5, 1, 2 J/cm2) and radiation periodicity of once, twice,...
Tue Mar 10, 2020 12:38
The author name Bruno Menfridini Baroni was incorrectly captured in the original article. The correct author name is Bruno Manfredini Baroni. The original article has been corrected.
Tue Mar 10, 2020 12:38
Abstract Primary hepatocellular carcinoma (HCC) and colon carcinoma are two of the most common clinical malignancies along with high morbidity and mortality. As low-power laser irradiation (LPLI) can induce cytotoxicity or cell apoptosis on several types of hyperplasia, LPLI may be a potential alternative treatment for gastroenterological cancers. The current in vitro study focused on LPLI-induced apoptosis and mechanism after 532-nm laser irradiation on two different carcinoma...
Tue Mar 10, 2020 12:38
Abstract Observe the influence of femtosecond laser cutting on corneal biomechanics during small-incision lenticule extraction (SMILE) or femtosecond-assisted laser in situ keratomileusis (FS-LASIK) and assess the biomechanical changes following the operation. Prospective, non-randomized study. A total of 80 eyes from 80 patients were treated with either SMILE or FS-LASIK. Parameters of inverse concave radius, deformation amplitude ratio 2 mm or 1 mm (DA ratio 2 mm or 1 mm),...
Tue Mar 10, 2020 12:38
Abstract To evaluate the effectiveness of an exercise program associated to photobiomodulation (PBM) on pain, postural changes, functionally, and muscular strength in women, one of the risk factors, with knee osteoarthritis (OA). A randomized controlled trial, with a blinded assessor and intention-to-treat analysis and placebo control. Sixty-two participants with knee OA (with confirmed radiological diagnosis) were evaluated for this study. However, 34 were considered eligible...
Tue Mar 10, 2020 02:00
Abstract Endoscopic management of benign biliary stricture (BBS) remains challenging. Stenting is currently used for BBS management, but refractory BBS remains problematic. The aim of this study was to assess the safety and feasibility of a dilation balloon-equipped cylindrical light diffuser for BBS in a large animal model. A total of seven mini-pigs were used in the current study. Laser settings were chosen based on the findings of a previous animal study. Five animals were...
Tue Mar 10, 2020 02:00
Publication date: Available online 9 March 2020Source: Cancer/RadiothérapieAuthor(s): J.-L. Dumas, M. Fawzi, H. Masset, S. Losa, R. Dal, N. Pierrat, L. de Marzi
Tue Mar 10, 2020 12:35
Related Articles[Clinical features of the attentional deficit and hyperactivity disorder in epilepsy]. Medicina (B Aires). 2020;80 Suppl 2:58-62 Authors: Venegas V, De Pablo JM, Olbrich C Abstract Epilepsy and attention deficit and hyperactivity disorder (ADHD) are frequent conditions in pediatrics. Their association is frequent and complex, often sharing psychiatric comorbidity. Patients who present epilepsy and ADHD, show equal frequency in both genders,...
Tue Mar 10, 2020 14:02
Related ArticlesHealth service costs and their association with functional impairment among adults receiving integrated mental health care in five low- and middle-income countries: the PRIME cohort study. Health Policy Plan. 2020 Mar 09;: Authors: Chisholm D, Garman E, Breuer E, Fekadu A, Hanlon C, Jordans M, Kathree T, Kigozi F, Luitel N, Medhin G, Murhar V, Petersen I, Rathod SD, Shidhaye R, Ssebunnya J, Patel V, Lund C Abstract This study examines...
Tue Mar 10, 2020 14:02
Related ArticlesTreatment Outcomes of Pediatric Status Epilepticus in a Tertiary Pediatric Intensive Care Unit. Pediatr Emerg Care. 2020 Mar 03;: Authors: Cavusoglu D, Sınmaz EE, Dundar NO, Can FK, Anil AB, Sarioglu B Abstract OBJECTIVE: Status epilepticus is associated with high rates of morbidity and mortality; thus, early diagnosis and proper treatment are crucial. We aimed to study the etiology, clinical features, and treatment among pediatric patients...
Tue Mar 10, 2020 14:02
Related Articles[Prognosis of benign childhood epilepsy with centrotemporal spikes: clinical utility of objective diagnostic criteria]. Rev Neurol. 2020 Mar 16;70(6):206-212 Authors: Ramos-Lizana J, Martínez-Espinosa G, Jiménez-Iniesta E, Aguirre-Rodríguez J Abstract INTRODUCTION: Studies about childhood epilepsy with centrotemporal spikes (CECTS), most of them retrospective, include patients with highly heterogeneous features. AIM: To investigate...
Tue Mar 10, 2020 14:02
Related ArticlesFunctional Imbalance of Glutamate- and GABAergic Neuronal Systems in the Pathogenesis of Focal Drug-Resistant Epilepsy in Humans. Bull Exp Biol Med. 2020 Mar 09;: Authors: Sazhina TA, Sitovskaya DA, Zabrodskaya YM, Bazhanova ED Abstract The mechanisms of the formation of pharmacological resistance in temporal focal epilepsy remain poorly understood, and effective treatment strategies that can suppress epileptogenesis do not currently...
Tue Mar 10, 2020 12:31
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