Publication date: 1 November 2020Source: Talanta, Volume 219Author(s): Annelise Genoux, Thibaut Duparc, Jean-Bernard Ruidavets, Cécile Ingueneau, Souad Najib, Jean Ferrières, Bertrand Perret, Mikaël Croyal, Laurent O. Martinez
Tue Jul 07, 2020 15:47
Infection with the bacteria Helicobacter pylori has been classified as class one carcinogen associated with increasing susceptibility of gastritis and gastric carcinoma. This study is aiming at investigating the ...
Mon Jul 06, 2020 03:00
Hyperthyroidism is the excessive synthesis of thyroid hormones. Thyroid uptake scans and ultrasonography provide an accurate diagnosis of hyperthyroidism, especially when thyroid receptor antibody (TRAb) measu...
Mon Jul 06, 2020 03:00
Naja ashei is a snake of medical importance in Kenya, Ethiopia, Somalia, Uganda, and Tanzania. Little is known about the enzymatic (snake venom phospholipases A2; svPLA2’s) and toxic (lethal) activities of N. ash...
Mon Jul 06, 2020 03:00
This study aimed to clarify the factors of successful inter-agency collaboration that affect multidisciplinary workers’ abilities to identify child maltreatment. A questionnaire-based survey was conducted; the...
Mon Jul 06, 2020 03:00
Related Articles [Management of patients with vestibular schwannoma type IV]. Laryngorhinootologie. 2020 Jul 06;: Authors: Harati A, Oni P, Schultheiß R, Deitmer T Abstract BACKGROUND: Vestibular schwannomas (VSs) are benign, slow-growing tumors that are classified by the Koos and Hannover grading scales. Despite extensive study, the management of large VS remains controversial. PATIENTS AND METHODS: Between 2003 and 2018, 61 patients...
Tue Jul 07, 2020 15:35
Related Articles Prognostic value of pre-operative peripheral inflammation markers in patients with squamous cell carcinoma of the external auditory canal. Braz J Otorhinolaryngol. 2020 Jun 24;: Authors: Li F, Shi X, Dai C Abstract INTRODUCTION: Squamous cell carcinoma of the external auditory canal is a rare entity. Previous studies have suggested predictors for tumor recurrence. However, most of the prognostic factors were from the clinicopathological...
Tue Jul 07, 2020 15:35
Publication date: Available online 6 July 2020Source: Gait & PostureAuthor(s): Daniela Kuchen, Patric Eichelberger, Heiner Baur, Erich Rutz
Tue Jul 07, 2020 15:35
Publication date: Available online 6 July 2020Source: Gait & PostureAuthor(s): Petros Ismailidis, Corina Nüesch, Mara Kaufmann, Martin Clauss, Geert Pagenstert, Anke Eckardt, Thomas Ilchmann, Annegret Mündermann
Tue Jul 07, 2020 15:35
Publication date: Available online 6 July 2020Source: Gait & PostureAuthor(s): Jennifer J. Bagwell, Nicholas Reynolds, Michelle Walaszek, Hannah Runez, Kristina Lam, Jo Armour Smith, Dimitrios Katsavelis
Tue Jul 07, 2020 15:35
Related Articles RNA-Seq Profiling of Serum Exosomal Circular RNAs Reveals Circ-PNN as a Potential Biomarker for Human Colorectal Cancer. Front Oncol. 2020;10:982 Authors: Xie Y, Li J, Li P, Li N, Zhang Y, Binang H, Zhao Y, Duan W, Chen Y, Wang Y, Du L, Wang C Abstract Circular RNAs (circRNAs) are emerging as cardinal areas of focus in the non-coding RNA field. Growing evidences have revealed exosomal circRNAs as potential biomarkers for detection...
Tue Jul 07, 2020 15:35
Related Articles Metabolomic Markers of Colorectal Tumor With Different Clinicopathological Features. Front Oncol. 2020;10:981 Authors: Long Z, Zhou J, Xie K, Wu Z, Yin H, Daria V, Tian J, Zhang N, Li L, Zhao Y, Wang F, Wang M, Cui Y Abstract Background: Colorectal cancer (CRC) is the result of complex interactions between the tumor's molecular profile and metabolites produced by its microenvironment. Despite recent studies identifying CRC molecular...
Tue Jul 07, 2020 15:35
Related Articles Long-Term Remission Achieved by Ponatinib and Donor Lymphocytes Infusion in a Ph+ Acute Lymphoblastic Leukemia Patient in Molecular Relapse After Allogenic Stem Cell Transplant and Dasatinib: A Case Report. Front Oncol. 2020;10:967 Authors: Botta C, Caruso N, Bossio S, Storino F, Console G, Martino M, Mendicino F, Lucia E, Morelli R, Correale P, Morabito F, Gentile M, Vigna E Abstract Currently, the prognosis of Ph+ acute lymphoblastic...
Tue Jul 07, 2020 15:35
Related Articles Heme-Dependent ER Stress Apoptosis: A Mechanism for the Selective Toxicity of the Dihydroartemisinin, NSC735847, in Colorectal Cancer Cells. Front Oncol. 2020;10:965 Authors: Elhassanny AEM, Soliman E, Marie M, McGuire P, Gul W, ElSohly M, Van Dross R Abstract Colorectal cancer (CRC) is a leading cause of cancer death in the United States. Artemisinin derivatives, including the dihydroartemisinin (DHA) monomers, are widely used...
Tue Jul 07, 2020 15:35
Related Articles Downregulated FOXO3a Associates With Poor Prognosis and Promotes Cell Invasion and Migration via WNT/β-catenin Signaling in Cervical Carcinoma. Front Oncol. 2020;10:903 Authors: Tian Y, Qi P, Hu X Abstract Background: Emerging studies have demonstrated that the Forkhead transcription factor FOXO3a is closely correlated with the progression of multiple tumors. Nevertheless, the biological role and prognostic value of FOXO3a have...
Tue Jul 07, 2020 15:35
Related Articles Imaging Features of Sclerosing Epithelioid Fibrosarcoma of the Pancreas: A Case Report. Front Oncol. 2020;10:901 Authors: Xia W, Yang Y, Huang Y Abstract Background: Sclerosing epithelioid fibrosarcoma (SEF) is an extremely rare fibrosarcoma variant. There is no complete imaging data on SEF involving the pancreas. Herein we report the computed tomography (CT) and magnetic resonance imaging (MRI) data of a patient with SEF that...
Tue Jul 07, 2020 15:35
Related Articles Protein Kinase CK2 in Cancer Energetics. Front Oncol. 2020;10:893 Authors: Silva-Pavez E, Tapia JC Abstract Protein kinase CK2 (formerly known as casein kinase 2) is abnormally elevated in many cancers. This may increase tumor aggressiveness through CK2-dependent phosphorylation of key proteins in several signaling pathways. In this work, we have compiled evidence from the literature to suggest that CK2 also modulates a metabolic...
Tue Jul 07, 2020 15:35
Related Articles The Potential of Tumor Debulking to Support Molecular Targeted Therapies. Front Oncol. 2020;10:801 Authors: Oppel F, Görner M, Sudhoff H Abstract Tumors may consist of billions of cells, which in malignant cases disseminate and form distant metastases. The large number of tumor cells formed by the high number of cell divisions during tumor progression creates a heterogeneous set of genetically diverse tumor cell clones. For...
Tue Jul 07, 2020 15:35
Related Articles Prognostic Value of Pre-Treatment Prognostic Nutritional Index in Esophageal Cancer: A Systematic Review and Meta-Analysis. Front Oncol. 2020;10:797 Authors: Hao J, Chen C, Wan F, Zhu Y, Jin H, Zhou J, Chen N, Yang J, Pu Q Abstract Background: Prognostic nutritional index (PNI), combining albumin and lymphocyte counts, which represent the nutritional and immune status, was considered as an effective predictor for the patient's...
Tue Jul 07, 2020 15:35
Related Articles Methylation Profile of X-Chromosome-Related Genes in Male Breast Cancer. Front Oncol. 2020;10:784 Authors: Foschini MP, Morandi L, Sanchez AM, Santoro A, Mulè A, Zannoni GF, Varga Z, Moskovszky L, Cucchi MC, Moelans CB, Giove G, van Diest PJ, Masetti R Abstract Background: Androgen receptor (AR) has been described to play a prominent role in male breast cancer (MBC). It maps on chromosome X, and recent reports indicate that...
Tue Jul 07, 2020 15:35
Early T Stage Is Associated With Poor Prognosis in Patients With Metastatic Liver Colorectal Cancer.
Related Articles Early T Stage Is Associated With Poor Prognosis in Patients With Metastatic Liver Colorectal Cancer. Front Oncol. 2020;10:716 Authors: Wu L, Fu J, Chen Y, Wang L, Zheng S Abstract Clinically, a considerable portion of patients with early T stage who were supposed to have a low distant metastatic probability were diagnosed with metastatic liver colorectal cancer (CRLM). Our study aims to evaluate the prognostic value of the T...
Tue Jul 07, 2020 15:35
Related Articles Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes. J Vis Exp. 2020 Jun 17;(160): Authors: Droho S, Cuda CM, Lavine JA Abstract The innate immune system plays important roles in ocular pathophysiology including uveitis, diabetic retinopathy, and age-related macular degeneration. Innate immune cells, specifically mononuclear phagocytes, express overlapping cell surface markers,...
Tue Jul 07, 2020 15:33
Related Articles A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. J Vis Exp. 2020 Jun 17;(160): Authors: Atta H, Gheinani AH, Wacker A, Heshmati Y, Bigger-Allen A, Lambrinos G, Gao Y, Bielenberg DR, Adam RM Abstract We describe the implementation of spinal cord injury in mice to elicit detrusor-sphincter dyssynergia, a functional bladder outlet obstruction, and subsequent...
Tue Jul 07, 2020 15:33
Related Articles Ubiquitin Chain Analysis by Parallel Reaction Monitoring. J Vis Exp. 2020 Jun 17;(160): Authors: Longworth J, Mendes ML, Dittmar G Abstract Assessment of the global profile of ubiquitin chain topologies within a proteome is of interest to answer a wide range of biological questions. The protocol outlined here takes advantage of the di-glycine (-GG) modification left after the tryptic digestion of ubiquitin incorporated...
Tue Jul 07, 2020 15:33
Related Articles Using a Thermal Camera to Measure Heat Loss Through Bird Feather Coats. J Vis Exp. 2020 Jun 17;(160): Authors: Graveley JMF, Burgio KR, Rubega M Abstract Feathers are essential to insulation, and therefore to the cost of thermoregulation, in birds. There is robust literature on the energetic cost of thermoregulation in birds across a variety of ecological circumstances. However, few studies characterize the contribution...
Tue Jul 07, 2020 15:33
Related Articles Electrocardiogram Recordings in Anesthetized Mice using Lead II. J Vis Exp. 2020 Jun 20;(160): Authors: Ha TW, Oh B, Kang JO Abstract The electrocardiogram is a valuable tool for evaluating the cardiac conduction system. Animal research has helped generate novel genetic and pharmacological information regarding the electrocardiogram. However, making electrocardiogram measurements in small animals in vivo, such as mice,...
Tue Jul 07, 2020 15:33
Related Articles Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica. J Vis Exp. 2020 Jun 16;(160): Authors: Masunaga A, Liu AW, Tan Y, Scott A, Luscombe NM Abstract Oikopleura dioica is a planktonic chordate with exceptional filter-feeding ability, rapid generation time, conserved early development, and a compact genome. For these reasons, it is considered a useful model organism for marine ecological...
Tue Jul 07, 2020 15:33
Related Articles A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules. J Vis Exp. 2020 Jun 20;(160): Authors: Zhang Y, Kurosawa K, Nishiura D, Tei M, Tsudome M Abstract Advances in spatial resolution and detection sensitivity of scientific instrumentation make it possible to apply small reactors for biological and chemical research. To meet the demand for high-performance microreactors, we developed...
Tue Jul 07, 2020 15:33
Related Articles Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging. J Vis Exp. 2020 Jun 20;(160): Authors: Knutsen RH, Gober LM, Sukinik JR, Donahue DR, Kronquist EK, Levin MD, McLean SE, Kozel BA Abstract Blood vessels form intricate networks in 3-dimensional space. Consequently, it is difficult to visually appreciate how vascular networks interact and behave by observing the surface of a tissue. This method...
Tue Jul 07, 2020 15:33
Related Articles Fabrication of Surface Acoustic Wave Devices on Lithium Niobate. J Vis Exp. 2020 Jun 18;(160): Authors: Mei J, Zhang N, Friend J Abstract Manipulation of fluids and particles by acoustic actuation at small scale is aiding the rapid growth of lab-on-a-chip applications. Megahertz-order surface acoustic wave (SAW) devices generate enormous accelerations on their surface, up to 108 m/s2, in turn responsible for many of the...
Tue Jul 07, 2020 15:33
Related Articles Manganese Oxide Nanoparticle Synthesis by Thermal Decomposition of Manganese(II) Acetylacetonate. J Vis Exp. 2020 Jun 18;(160): Authors: Martinez de la Torre C, Bennewitz MF Abstract For biomedical applications, metal oxide nanoparticles such as iron oxide and manganese oxide (MnO), have been used as biosensors and contrast agents in magnetic resonance imaging (MRI). While iron oxide nanoparticles provide constant negative...
Tue Jul 07, 2020 15:33
Related Articles In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes. J Vis Exp. 2020 Jun 22;(160): Authors: Gonçalves-Rodrigues P, Almeida-Coelho J, Gonçalves A, Amorim F, Leite-Moreira AF, Stienen GJM, Falcão-Pires I Abstract In this article, we describe the steps required to isolate a single permeabilized ("skinned") cardiomyocyte and attach it to a force-measuring apparatus and a motor to perform functional studies....
Tue Jul 07, 2020 15:33
Related Articles An Externally-Heated Diamond Anvil Cell for Synthesis and Single-Crystal Elasticity Determination of Ice-VII at High Pressure-Temperature Conditions. J Vis Exp. 2020 Jun 18;(160): Authors: Lai X, Zhu F, Zhang JS, Zhang D, Tkachev S, Prakapenka VB, Chen B Abstract The externally-heated diamond anvil cell (EHDAC) can be used to generate simultaneously high-pressure and high-temperature conditions found in Earth's and planetary...
Tue Jul 07, 2020 15:33
Related Articles Using a Chemical Biopsy for Graft Quality Assessment. J Vis Exp. 2020 Jun 17;(160): Authors: Stryjak I, Warmuzińska N, Łuczykowski K, Hamar M, Urbanellis P, Wojtal E, Masztalerz M, Selzner M, Włodarczyk Z, Bojko B Abstract Kidney transplantation is a life-saving treatment for a large number of people with end-stage renal dysfunction worldwide. The procedure is associated with an increased survival rate and greater quality...
Tue Jul 07, 2020 15:33
Related Articles Evaluation of Color Difference in Placenta with Twin Anemia Polycythemia Sequence. J Vis Exp. 2020 Jun 17;(160): Authors: Zhao D, Tollenaar LSA, Slaghekke F, Oepkes D, Duan T, Lopriore E Abstract Twin anemia polycythemia sequence (TAPS) occurs in 5% of monochorionic twins and is characterized by large inter-twin hemoglobin differences. The postnatal diagnostic criteria for TAPS are based on hematologic parameters and placental...
Tue Jul 07, 2020 15:33
Related Articles Production of Germ-Free Fast-Growing Broilers from a Commercial Line for Microbiota Studies. J Vis Exp. 2020 Jun 18;(160): Authors: Guitton E, Faurie A, Lavillatte S, Chaumeil T, Gaboriaud P, Bussière F, Laurent F, Lacroix-Lamandé S, Guabiraba R, Schouler C Abstract Studies of the gut microbiota contribution to the host physiology and immunocompetence are facilitated by the availability of germ-free animal models, which...
Tue Jul 07, 2020 15:33
Related Articles Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis. J Vis Exp. 2020 Jun 18;(160): Authors: O'Sullivan KM, Creed S, Gan PY, Holdsworth SR Abstract Glomerular cell death is a pathological feature of myeloperoxidase anti neutrophil cytoplasmic antibody associated vasculitis (MPO-AAV). Extracellular deoxyribonucleic acid (ecDNA) is released during different forms of cell death including...
Tue Jul 07, 2020 15:33
Related Articles Analysis of Human Natural Killer Cell Metabolism. J Vis Exp. 2020 Jun 22;(160): Authors: Traba J, Waldmann TA, Anton OM Abstract Natural Killer (NK) cells mediate mainly innate anti-tumor and anti-viral immune responses and respond to a variety of cytokines and other stimuli to promote survival, cellular proliferation, production of cytokines such as interferon gamma (IFNγ) and/or cytotoxicity programs. NK cell activation...
Tue Jul 07, 2020 15:33
Related Articles High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon. J Vis Exp. 2020 Jun 16;(160): Authors: Matsuda A, Koujin T, Schermelleh L, Haraguchi T, Hiraoka Y Abstract Quantitative multicolor fluorescence microscopy relies on the careful spatial matching of color channels acquired at different wavelengths. Due to chromatic aberration and the imperfect alignment of cameras,...
Tue Jul 07, 2020 15:33
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