Abstract Background The relationship of airway hyperresponsiveness to airway remodeling and inflammation in infants with wheeze is unclear. Objective To investigate airway hyperresponsiveness, remodeling and inflammation in infants with wheeze and troublesome breathing. Methods Inclusion criteria: full‐term, 3‐23 months of age; doctor diagnosed wheeze and persistent recurrent troublesome breathing; without obvious structural defect, suspicion of ciliary dyskinesia, cystic...
Wed Mar 11, 2020 22:20
Here, we characterize protein structure and interaction sites in living cells using a protein footprinting technique termed in-cell fast photochemical oxidation of proteins (IC-FPOP).
Wed Mar 11, 2020 21:50
We describe a method of inducing hairy roots by Agrobacterium rhizogenes-mediated transformation in Tartary buckwheat (Fagopyrum tataricum). This can be used to investigate gene functions and production of secondary metabolites in Tartary buckwheat, be adopted for any genetic transformation, or used for other medicinal plants after improvement.
Wed Mar 11, 2020 20:49
We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow cell.
Wed Mar 11, 2020 20:49
Here, we present a versatile method for tomato root transformation followed by inoculation with Ralstonia solanacearum to perform straightforward genetic analysis for the study of bacterial wilt disease.
Wed Mar 11, 2020 19:48
The mammary gland is a bilayered structure, comprising outer myoepithelial and inner luminal epithelial cells. Presented is a protocol to prepare organoids using differential trypsinization. This efficient method allows researchers to separately manipulate these two cell types to explore questions concerning their roles in mammary gland form and function.
Wed Mar 11, 2020 19:48
We present a protocol to evaluate the balance between glutamate release and clearance at single corticostriatal glutamatergic synapses in acute slices from adult mice. This protocol uses the fluorescent sensor iGluu for glutamate detection, a sCMOS camera for signal acquisition and a device for focal laser illumination.
Wed Mar 11, 2020 18:48
A protocol for the space payload design, the space experiment on thermocapillary convection, and analyses of experimental data and images are presented in this paper.
Wed Mar 11, 2020 18:48
Reprocessing reusable medical devices is crucial in the healthcare industry. To ensure patient safety, strict standards are dictated to validate thermal disinfection in automated washer-disinfectors. The Unite...
Tue Mar 10, 2020 02:00
Trichosporon asahii is the major causative fungus of disseminated or deep-seated trichosporonosis and forms a biofilm on medical devices. Biofilm formation leads to antifungal drug resistance, so biofilm-related ...
Tue Mar 10, 2020 02:00
Timely implementation of influenza infection control and treatment can significantly reduce the impact on Hospital resources and patient management when demand is at peak. Turnaround times of Laboratory based ...
Tue Mar 10, 2020 02:00
Blue light has been attributed to the adverse biological effects caused by the use of smartphones and tablet devices at night. However, it is not realistic to immediately avoid nighttime exposure to blue light...
Tue Mar 10, 2020 02:00
During the Middle Jurassic Period, the Isle of Skye in Scotland was home to a thriving community of dinosaurs that stomped across the ancient coastline, according to a new study.
Wed Mar 11, 2020 20:05
Scientists have used CRISPR technology to genetically engineer rice with high levels of beta-carotene, the precursor of vitamin A. The technique they used provides a promising strategy for genetically improving rice and other crops.
Thu Mar 05, 2020 20:20
Abstract Objective The aim of this study was to evaluate the long-term outcomes of initial Gamma Knife radiosurgery (GKRS) for patients with nonfunctioning pituitary adenomas (NFPAs). Design and methods This was a single-center retrospective study. Eighty-one patients with NFPAs undergoing initial GKRS were enrolled. The median age was 44.9 years (range,...
Wed Mar 11, 2020 02:00
Abstract Magnetic resonance (MR) imaging is an essential tool in the diagnosis and management of pituitary diseases, indispensable for making correct treatment decisions. Successful management and follow-up of pituitary pathology requires an understanding of the MR appearance of normal and abnormal structures in the sellar region. This review will describe the MR appearance of the normal and abnormal pituitary gland and proposes an algorithm for the management strategy of some...
Wed Mar 11, 2020 02:00
Abstract Familial nonmedullary thyroid cancer (FNMTC) constitutes 3–9% of all thyroid cancer cases. FNMTC is divided into two groups: syndromic and nonsyndromic. Nonsyndromic FNMTC is more common as compared with syndromic FNMTC. In syndromic FNMTC, patients are at risk of nonmedullary thyroid cancer (NMTC) and other tumors, and the susceptibility genes are known. In nonsyndromic FNMTC, NMTC is the major feature of the disease and occurs in isolation with an autosomal dominant...
Wed Mar 11, 2020 02:00
Wed Mar 11, 2020 02:00
The field of prosthetics has been evolving and advancing over the past decade, as patients with missing extremities are expecting to control their prostheses in as normal a way as possible. Scientists have att...
Tue Mar 10, 2020 02:00
Wed Mar 11, 2020 20:23
Publication date: Available online 10 March 2020Source: International Journal of Oral and Maxillofacial SurgeryAuthor(s): G. Dot, F. Rafflenbeul, M. Arbotto, L. Gajny, P. Rouch, T. Schouman
Wed Mar 11, 2020 20:06
Publication date: Available online 10 March 2020Source: Archives of Physical Medicine and RehabilitationAuthor(s): Susan L. Kasser, Asim Zia
Wed Mar 11, 2020 19:49
To compare treatment plans for interstitial high dose rate (HDR) liver brachytherapy with 192Ir calculated according to current-standard TG-43U1 protocol with model-based dose calculation following TG-186 protoco...
Mon Mar 09, 2020 02:00
Abstract Background Studies have indicated that bacterial vaginosis (BV) might be a cofactor for the acquisition and persistence of high‐risk papillomavirus, enabling the development of cytological abnormalities. The presence of endocervical and metaplastic cells makes the smear more adequate for the detection of these abnormalities once these cell types are representative of the transformation zone, a site of increased susceptibility to viral infection. Methods The purpose of this...
Wed Mar 11, 2020 19:39
Publication date: Available online 11 March 2020Source: NeuroImageAuthor(s): Laurent Caplette, Frédéric Gosselin, Martial Mermillod, et Bruno Wicker
Wed Mar 11, 2020 19:18
Publication date: Available online 10 March 2020Source: NeuroImageAuthor(s): Rajapillai L.I. Pillai, Elizabeth A. Bartlett, Mala R. Ananth, Chencan Zhu, Jie Yang, Greg Hajcak, Ramin V. Parsey, Christine DeLorenzo
Wed Mar 11, 2020 19:18
Coronavirus disease 2019 (COVID-19) is optimized to spread widely: Its signs and symptoms are largely indistinguishable from those of other respiratory viruses. This commentary specifically addresses best ways to protect our hospitals against COVID-19.
Wed Mar 11, 2020 02:00
Estimates suggest that COVID-19 will stress bed capacity, equipment, and health care personnel in U.S. hospitals in ways not previously experienced. How can health systems prepare to care for a large influx of patients with this disease?
Wed Mar 11, 2020 02:00
Background:Apixaban and rivaroxaban are the most commonly prescribed direct oral anticoagulants for adults with atrial fibrillation, but head-to-head data comparing their safety and effectiveness are lacking.Objective:To compare the safety and effectiveness of apixaban versus rivaroxaban for patients with nonvalvular atrial fibrillation.Design:New-user, active-comparator, retrospective cohort study.Setting:A U.S. nationwide commercial health care claims database from 28 December 2012 to 1 January...
Tue Mar 10, 2020 02:00
Background:A novel human coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was identified in China in December 2019. There is limited support for many of its key epidemiologic features, including the incubation period for clinical disease (coronavirus disease 2019 [COVID-19]), which has important implications for surveillance and control activities.Objective:To estimate the length of the incubation period of COVID-19 and describe its public health implications.Design:Pooled...
Tue Mar 10, 2020 02:00
Tue Mar 10, 2020 02:00
Background:FLU-v is a broad-spectrum influenza vaccine that induces antibodies and cell-mediated immunity.Objective:To compare the safety, immunogenicity, and exploratory efficacy of different formulations and dosing regimens of FLU-v versus placebo.Design:Randomized, double-blind, placebo-controlled, single-center phase 2b clinical trial. (ClinicalTrials.gov: NCT02962908; EudraCT: 2015-001932-38)Setting:The Netherlands.Participants:175 healthy adults aged 18 to 60 years.Intervention:0.5-mL subcutaneous...
Tue Mar 10, 2020 02:00
The prognosis of patients with high‐grade or advanced‐stage endometrial cancer remains poor. As cancer stem‐like cells (CSCs) are thought to be associated with endometrial cancers, it is essential to investigate the molecular mechanisms that regulate endometrial CSCs. Dual specificity phosphatase 6 (DUSP6) functions as a negative‐feedback regulator of MAPK–ERK1/2 signaling, but its role in endometrial cancer remains unknown. We investigated whether DUSP6 is involved in cancer cell stemness using...
Wed Mar 11, 2020 18:55
Abstract Prostate cancer is a heterogeneous disease and optimum gene targeting treatment is often impermissible. We aim to determine the intratumoral genomic heterogeneity of prostate cancer and explore candidate genes for targeted therapy. Exome sequencing was performed on 37 samples from 16 patients with prostate cancer. Somatic variant analysis, copy number variant (CNV) analysis, clonal evolution analysis and variant spectrum analysis were used to study the intratumoral genomic heterogeneity...
Wed Mar 11, 2020 18:55
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