Abstract Dyeing methods that conform to environmental protection requirements and adapt to the development of industry are investigated using bleached poplar wood dyed with melanin secreted by Lasiodiplodia theobromae in this paper. Prolonging the dyeing time of coarse melanin can improve the dye uptake of veneers, and the brightness value increases, and the reflectivity declines with the increase of dyeing time. The stained surface of the vessel is gradually covered by coarse...
Mon Mar 23, 2020 21:15
Abstract Ansamitocin P-3 (AP-3), a 19-membered polyketide macrocyclic lactam, has potent antitumor activity. Our previous study showed that a relatively low organic nitrogen concentration in culture medium could significantly improve AP-3 production of Actinosynnema pretiosum. In the present study, we aimed to reveal the possible reasons for this improvement through metabolomic and gene transcriptional analytical methods. At the same time, a metabolic pathway profile based on...
Mon Mar 23, 2020 20:15
Abstract Recent studies have shown that inflammatory diseases are becoming more frequent throughout the world. The causes of these disorders are multifactorial and include genetic, immunological, and environmental factors, and intestinal microbiota dysbiosis. The use of beneficial microorganisms has shown to be useful in the prevention and treatment of disorders such as colitis, mucositis, and even colon cancer by their immune-stimulating properties. It has also been shown that...
Mon Mar 23, 2020 20:15
Abstract Over the past few decades, Escherichia coli (E. coli) remains the most favorable host among the microbial cell factories for the production of soluble recombinant proteins. Recombinant protein production (RPP) via E. coli is optimized at the level of gene expression (expression level) and the process condition of fermentation (process level). Presently, the reported studies do not give a clear view on the selection of methods employed in the optimization of RPP. Here,...
Mon Mar 23, 2020 20:15
Abstract Single-molecule real-time (SMRT) sequencing can be used to identify a wide variety of chemical modifications of the genome, such as methylation. Here, we applied this approach to identify N6-methyl-adenine (m6A) and N4-methyl-cytosine (m4C) modification in the genome of Bacillus pumilus BA06. A typical methylation recognition motif of the type I restriction-modification system (R-M), 5′-TCm6AN8TTGG-3′/3′-AGTN8m6AACC-5′, was identified. We confirmed that this motif was...
Mon Mar 23, 2020 20:15
Abstract γ-Glutamylcysteine synthetase (γ-GCS) from Escherichia coli, which catalyzes the formation of l-glutamylcysteine from l-glutamic acid and l-cysteine, was engineered into an l-theanine synthase using l-glutamic acid and ethylamine as substrates. A high-throughput screening method using a 96-well plate was developed to evaluate the l-theanine synthesis reaction. Both site-saturation mutagenesis and random mutagenesis were applied. After three rounds of directed evolution,...
Mon Mar 23, 2020 20:15
Abstract Alginate oligosaccharides are associated with some beneficial health effects. Gut microbiota is one of the most recently identified factors in the development of several metabolic diseases induced by high-fat diet. Our objective was to evaluate how alginate oligosaccharides impact on high-fat diet‑induced features of metabolic disorders and whether this impact is related to modulations in the modulation of the gut microbiota. C57BL/6J mice were fed with chow diet, high-fat...
Mon Mar 23, 2020 20:15
Abstract Even though they are among the most extreme environments in which life can develop, glaciers are colonized by metabolically active microbes, some of which thrive—in their own particular way—under the prevailing harsh conditions. Glacial or periglacial microbes are often psychrophiles since they are able to grow optimally at low temperatures. This ability has evolved through a series of adaptations, both molecular and physiological, some of which have been exploited...
Mon Mar 23, 2020 20:15
Abstract Bacterial non-specific nucleases are ubiquitously distributed and involved in numerous intra- and extracellular processes. Although all nucleases share the basic chemistry for the hydrolysis of phosphodiester bonds in nucleic acid molecules, the catalysis comprises diverse modes of action, which offers great potential for versatile biotechnological applications. A major criterium for their differentiation is substrate specificity. Specific endonucleases are widely used...
Mon Mar 23, 2020 20:15
Abstract In vitro models of angiogenesis are valuable tools for understanding the underlying mechanisms of pathological conditions and for the preclinical evaluation of therapies. Our laboratory developed the rat mesentery culture model as a new tool for investigating mechanistic cell–cell interactions at specific locations across intact blood and lymphatic microvascular networks ex vivo. The objective of this study was to report a method for evaluating the effect of aging on human...
Mon Mar 23, 2020 02:00
In vivo imaging is a powerful tool that can be used to investigate the cellular mechanisms underlying nervous system development. Here we describe a technique for using time-lapse confocal microscopy to visualize large numbers of multicolor Brainbow-labeled cells in real time within the developing zebrafish nervous system.
Mon Mar 23, 2020 20:52
We present a method for the purification, detection, and identification of diGly peptides that originate from ubiquitinated proteins from complex biological samples. The presented method is reproducible, robust, and outperforms published methods with respect to the level of depth of the ubiquitinome analysis.
Mon Mar 23, 2020 19:51
Conditions: PTSD; Post-traumatic Stress DisorderIntervention: Behavioral: Deep Brain Reorienting (DBR)Sponsors: Lawson Health Research Institute; Canadian Institutes of Health Research (CIHR)Not yet recruiting
Mon Mar 23, 2020 18:00
Conditions: Postural; Defect; Stomatognathic System Abnormalities; MalocclusionInterventions: Diagnostic Test: Research Diagnostic Criteria of Functional Disorders of the Masticatory System BKD / ZCURNŻ - Polish version; Device: Clinical examination of the stomatognathic system (structural and functional); Device: 3D examination of the body posture; Device: Functional assessment of the spine and lower limbs; Device: Complementary testsSponsor: University of RzeszowNot yet recruiting
Mon Mar 23, 2020 18:00
Conditions: Neck Pain; PHYSIOTHERAPY; JAWInterventions: Other: Jaw opening-closing movements; Other: Specific Active exercises; Other: Exercise Therapy; Other: Supportive TreatmentSponsor: University of LahoreRecruiting
Mon Mar 23, 2020 18:00
Epistaxis, or nasal bleeding, occurs in over half of the general population. It is caused by various etiological factors and affects both sexes and all age groups. The simplest treatment for a nosebleed is pinching of the ala nasi, referred to as the Hippocratic technique. In this study, we adopted different treatment protocols dependent on the severity of bleeding and assessed the etiology and efficacy of these modalities. This was a prospective study. We recruited 25 patients (24 adults and 1 child)...
Mon Mar 23, 2020 10:20
Abstract Background The gastro-omental artery (GOA) and the greater omentum are nowadays commonly used in many reconstructive surgeries, including cardiac, vascular, and plastic surgery. There are cases in which the GOA is found to be unsuitable only after partial harvesting following an upper laparotomy, and an improved method of pre-operative evaluation is required to eliminate the need for intraoperative small laparotomy. ...
Mon Mar 23, 2020 02:00
Abstract Endometriosis constitutes the presence of ectopic endometrial glands and stroma outside the uterine endometrium, which is hormonally responsive and responds to pregnancy hormones as well. Decidualization is a physiologic process, where the normal endometrium readies itself for optimal accommodation of a pregnancy. A similar hormonal response can be seen with ectopic endometrium as well. As such, ovarian endometriomas and deep endometriosis implants can undergo decidualization....
Mon Mar 23, 2020 02:00
Abstract Background Carbon ion radiotherapy (CIRT) is an emerging radiation therapy to treat skull base chordomas and chondrosarcomas. To date, its use is limited to a few centers around the world, and there has been no attempt to systematically evaluate survival and toxicity outcomes reported in the literature. Correspondingly, the aim of this study was to qualitatively and quantitatively assess these outcomes. ...
Mon Mar 23, 2020 02:00
Abstract Introduction Standard of care for glioblastoma includes concurrent chemoradiation and maintenance temozolomide with tumor treatment fields (TTFields). Preclinical studies suggest TTFields and radiation treatment have synergistic effects. We report our initial experience evaluating toxicity and tolerability of scalp-sparing radiation with concurrent TTFields. Methods...
Mon Mar 23, 2020 02:00
Abstract ATP-binding cassette (ABC) transporters constitute a superfamily of 48 structurally similar membrane transporters that mediate the ATP-dependent cellular export of a plethora of endogenous and xenobiotic substances. Importantly, genetic variants in ABC genes that affect gene function have clinically important effects on drug disposition and can be predictors of the risk of adverse drug reactions and efficacy of chemotherapeutics, calcium channel blockers, and protease...
Mon Mar 23, 2020 02:00
Ibrutinib is a major new addition to the therapeutic armamentarium for chronic lymphocytic leukemia, mantle cell lymphoma, Waldenstrom’s macroglobulinemia, and chronic graft versus host disease. Though ibrutinib has proven to be a revolutionary new small molecule agent, and has relatively minimal toxicity as compared to traditional chemotherapy, infections have emerged as a major complication of therapy. While fungal infections have been the most problematic (including CNS aspergillosis), zoster,...
Mon Mar 23, 2020 18:20
Abstract Background We previously have shown that there was a strong correlation between failed facemask ventilation, failed ventilation through a supraglottic airway, and difficult tracheal intubation. The primary aim of this study was to evaluate whether or not an established method to predict difficult ventilation through a supraglottic airway was also useful for predicting failed facemask ventilation. ...
Mon Mar 23, 2020 02:00
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου