Κυριακή 5 Απριλίου 2020


Contribution of Hydrogen Sulfide to Dilation of Rat Cerebral Arteries after Ischemia/Reperfusion Injury
The study examined the effect of H2S on the tone of cerebral arteries in rats after global cerebral ischemia/reperfusion injury and cooperation between NO and H2S in the control over cerebral circulation during the postischemic period. In control sham-operated and in experimental rats with ischemia/reperfusion injury, the diameter of pial arteries was repeatedly measured in vivo under a light microscope after removal of parietal bone and dura mater in 1 h and in 7 days after the surgery. The study...
Latest Results for Bulletin of Experimental Biology and Medicine
2h
Ammonium Salts Increase Physical Performance and Reduce Blood Lactate Level in Rats in a Model of Forced Swimming
We compared the effects of two doses of ammonium chloride and ammonium carbonate (10 and 20 mg/kg) on the duration of swimming and blood lactate level. Ammonium chloride in a dose of 20 mg/kg was more efficient than in a dose of 10 mg/kg. The efficiency of ammonium carbonate in a dose of 10 mg/kg was similar to that of ammonium chloride in a dose of 20 mg/kg. Increasing the dose of ammonium carbonate to 20 mg/kg led to a decrease in the duration of swimming. On the last day of the experiment, lactate...
Latest Results for Bulletin of Experimental Biology and Medicine
2h
Effect of Infrared Low-Intensity Laser Irradiation on Lipid Peroxidation under Conditions of Experimental Circulatory Hypoxia of Visual Analyzer
The physiological stress modeled by circulatory hypoxia activates LPO processes in various tissues. In posthypoxic period, the infrared low-intensity laser irradiation significantly decreased the chemiluminescence parameters in blood plasma, normalized the retinal levels of diene and triene conjugates, and decreased MDA in the rat brain attesting to the correcting effect of this irradiation during various types of physiological stresses.
Latest Results for Bulletin of Experimental Biology and Medicine
2h
The Relationship of Immunoexpression of Ki-67 and Hsp70 with Clinical and Morphological Parameters and Prognosis of Papillary Thyroid Cancer
We studied the correlations of the expression of Ki-67 proliferation marker and localization of heat shock protein Hsp70 in tumor cells of papillary thyroid cancer with some clinical and morphological parameters and a 10-year survival prognosis. High variability of the proliferative activity (by Ki-67 index) of tumor cells was revealed. It was found that Hsp70 protein has different localization in tumor cells: cytoplasmic, nuclear, nucleolar, and mixed. Nuclear translocation of the Hsp70 protein...
Latest Results for Bulletin of Experimental Biology and Medicine
1d
Cytokines of the Lymph and Blood Serum as Markers of Oncogenesis and Efficiency of Therapy in Chemically Induced Breast Cancer in Wistar Rats
We studied the levels of serum and lymph cytokines involved in the pathogenesis of BC. BC was induced by injection of N-methyl-N-nitrosourea to Wistar rats. The animals underwent surgery, or received polychemotherapy (cyclophosphamide, methotrexate, and 5-fluorouracil), or surgical treatment was combined with polychemotherapy; in a special group, Panagen (fragmented DNA) was added to polychemotherapy. Cytokine concentration in the lymph was measured using Bio-Plex Pro Rat Cytokoness 24-Plex Assay...
Latest Results for Bulletin of Experimental Biology and Medicine
1d
Prognostic Role of Matrix Metalloproteinases 2, 7, 8, 9 and Their Type 1 Tissue Inhibitor in Blood Serum of Patients with Kidney Cancer
The study compared the levels of MMP-2,7,8,9, and TIMP-1 in blood serum of healthy people (N=97) and patients with primary renal cell carcinoma (N=93) to assess relevance of these markers to prognosis of overall survival of these patients, which were followed-up over 1 to 45 months (median 26 months). To evaluate the survival with the Kaplan—Meier estimator, the median values of examined markers in the total group of patients were taken as the threshold levels. This estimator showed that the high...
Latest Results for Bulletin of Experimental Biology and Medicine
1d
Genetic Basis of Attenuation of Cold-Adapted Influenza Strain B/Leningrad/14/17/55 — Backup Master Donor Virus for Influenza Type B Live Attenuated Vaccines
The reassortant vaccine strain of live attenuated influenza vaccine inherits temperature sensitivity and areactogenicity from cold-adapted attenuated master donor virus. In Russia, B/ USSR/60/69 master donor virus (B60) is currently in use for the preparation of live attenuated type B influenza vaccine candidates. Trivalent live attenuated influenza vaccine based on A/ Leningrad/134/17/57 and B60 are licensed for the use in Russia for single dose vaccination of adults and children over 3 years. B/Leningrad/14/17/55...
Latest Results for Bulletin of Experimental Biology and Medicine
1d
Morphological Study of Mesenteric Lymph Nodes in Rats with Experimental Breast Cancer Treated with Fragmented Double-Stranded DNA
We performed a morphometric analysis of mesenteric lymph nodes in rats with breast cancer induced by administration of N-methyl-N-nitrosourea. The volume of the paracortical zone and the number of mature plasma cells in the medullary sinuses were increased and the volume of lymphoid nodules with germinal centers and the number of macrophages were decreased in the group with tumor resection and chemotherapy in comparison with untreated rats with breast cancer. In rats receiving fragmented human double-stranded...
Latest Results for Bulletin of Experimental Biology and Medicine
1d
Peculiarities of Histological Structure of Pulmonary Veins in Patients with Total Anomalous Pulmonary Venous Drainage as Morphological Substrates Relating to Formation of Postoperative Pulmonary Venous Obstruction
The histological and immunohistochemical methods were employed to examine the peculiarities of histological structure of pulmonary veins and left atrium of the heart in norm and in various types of total anomalous drainage of pulmonary veins. In contrast to normal pulmonary vein covered with external multiple muscle layers (myocardial sleeve), such sleeve is absent in veins that have no connection with the left atrium irrespective of the type of the defect. In patients with total anomalous pulmonary...
Latest Results for Bulletin of Experimental Biology and Medicine
1d
Structural and Functional Changes in the Mesenteric Lymph Nodes in Humans during Aging
Morphometric analysis of structural and functional changes in the human mesenteric lymph nodes during aging revealed the development of fibrous connective tissue, fragmentation of the lymphoid parenchyma, the absence of follicles with germinal centers, and a decrease in the level of lymphocyte proliferation, which was confirmed by the absence of Ki-67+ cells. The paracortical zone lacked CD4+ T helpers that regulate both cellular and humoral immunity. High content of plasma cells and eosinophilic...
Latest Results for Bulletin of Experimental Biology and Medicine
1d
Beta-Catenin in Non-Small Cells Lung Cancer and Its Association with Proteasomes
We analyzed the content of β-catenin fractions and activity and content of proteasomes in the tissues of patients with non-small cells lung cancer. The content of β-catenin fractions was elevated and proteasome-dependent proteolysis in the tumor tissue was enhanced in comparison with the corresponding unchanged lung tissue. A negative regression relationship of caspase-like activity of proteasomes and a positive correlation between the content of proteasomes and both fractions of β-catenin were found....
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Energy Metabolism in Cellular Regenerative Processes: Focus on PPARα
Reduced expression of the key regulator of cardiac metabolism, transcription factor PPARα, in surgical samples of the auricles from patients with coronary heart disease and heart failure was detected by real-time quantitative PCR. These changes indicate reduced activity of this factor and a shift of energy metabolism from oxidative phosphorylation to glycolysis typical of dedifferentiated cells. Electron microscopy revealed dedifferentiated cardiomyocytes with disassembled contractile apparatus and...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Cardioprotective Effects of Metalloproteinase Inhibitor 1-({4-[(4-Chlorobenzoyl)amino]phenyl}sulfonyl-L-Proline in Modeled Acute Myocardial Infarction
Cardioprotective effect of 1-({4 [(4 chlorobenzoyl)amino]phenyl}sulfonyl-L-proline (compound AL-828) was studied in rats with modeled acute myocardial infarction. The test compound was administered intragastrically in a dose of 30 mg/kg/day for 3 days prior to infarction modeling. Metalloproteinase inhibitor antibiotic doxycycline served as the reference drug and was administered in a dose of 40 mg/kg/day by the same schedule. It was shown that AL-828 similar to doxycycline significantly reduced...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Mechanism of Natriuretic Effect of Oxytocin
In rats, intramuscular injection of oxytocin (0.25 nmol/100 g body weight) increased sodium excretion from 19±5 to 120±11 μmol/min. A significant correlation (p<0.001) was revealed between renal excretion of oxytocin and sodium ions. Under the action of oxytocin, natriuresis was characterized by diminished reabsorption of fluid in the proximal tubule of the nephron attested by elevated lithium clearance rate and from stimulation of V1a receptors in the cells of thick ascending loop of Henle. Pmp-Tyr(Me)-Phe-Gln-Asn-Cys-Pro-Arg-Gly-NH2,...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Differential Expression of Local Immune Response Genes in the Vagina: Implication for the Diagnosis of Vaginal Infections
Transcription profiles of genes of local immune response were determined in the vagina of women with bacterial vaginosis, aerobic vaginitis, and vulvovaginal candidosis for detection of the most specific immune markers for these vaginal infections. Laboratory diagnosis of the vaginal infections was performed microscopically; the inflammatory reaction in the vagina (leukorrhea) was defined as the presence of >10 white blood cells per field of view. Transcription profiles of IL1b, IL10, IL18, TNFα,...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Effect of Rheological Conditions of the Environment on Adaptive Behavior of Lactobacilli in Microculture
Adaptive behavior of lactobacilli in cultured in microchambers of different design was analyzed under a light microscope. We found that the time of appearance of first-generation cells for the studied strains of lactobacilli differed in chambers with different rheological properties (stationary and flow-through). The results of our experiments suggest that the development of populations of lactobacilli is regulated by autometabolites of different physiological modalities directly from the very first...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Acclimatization to Middle Attitude Hypoxia Masks the Symptoms of Experimental Posttraumatic Stress Disorder, but Does Not Affect Its Pathogenetic Mechanisms
The effects of acclimatization to middle attitude hypoxia on the resistance to acute emotional stress were studied on the model of posttraumatic stress disorder in rats. Anxyolitic, but not anxiogenic effect was observed in acclimatized rats. However, acclimatized rats with posttraumatic stress disorder were characterized by hypofunction of the pituitary—adrenocortical axis, which is typical of this pathology, and reduction in corticosterone/dehydroepiandrosterone ratio. At the neuroendocrine level,...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Correction of Lipid Metabolism Disorders in Diabetes Mellitus with Peptide Drugs
We studied the effect of peptide drugs deltalicin and Semax on lipid metabolism disturbances in diabetes mellitus. Diabetes mellitus was modeled by single injection of streptozotocin (45 mg/kg) and rats with blood glucose ≥12 mmol/liter were selected for the further experiments. Deltalicin in a dose 100 μg/kg and Semax in a dose 200 μg/kg as well as sulodexide corrected lipid metabolism disorders: the content of total cholesterol, triglycerides, LDL, index of atherogenicity decreased and HDL concentration...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Effects of Humic Acids, Isolated from High-Moor Pine-Peat Moss-Cotton Grass Peat on the Production of Cytokines by Mouse and Human Immunocompetent Cells and on Humoral Immune Response
A course of treatment with humic acids extracted with sodium pyrophosphate from high-moor pine-peat moss-cotton grass peat improves humoral immune response of C57BL/6 mice, stimulates the production of TNFα, IL-1β, and IL-12 by the animal peritoneal macrophages and the production of IFNγ and TNFα by donor peripheral blood mononuclears, causing no changes in the production of IL-10 in vitro.
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Inhibitory Activity of Scutellaria baicalensis Flavonoids against Tick-Borne Encephalitis Virus
We studied virus-inhibiting activity of Baikal skullcap (Scutellaria baicalensis) flavonoids against tick-borne encephalitis virus using various model schemes. The half-maximum cytotoxic concentration (CC50) for the plant extract was found (363.9±58.6 μg/ml). Based on the CC50 and IC50, selective index (SI) was calculated for viricidal (53.4), preventive (50.5), and direct antiviral actions (39.1) and for-intracellular replication of the virus (40.4). Suppression of virus reproduction ≥2.0 lg TCID50...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Effects of Catechin on Activity of Angiotensin-Converting Enzyme and Generation of Reactive Oxygen Species in Rat Aorta
Activity of angiotensin-converting enzyme (ACE) and intensity of ROS generation in the aorta were studied in male Wistar rats intraperitoneally injected with a mixture of (+) and (-) stereoisomers of catechin. ACE activity in aortal segments was evaluated by hydrolysis of hippuryl-histidine-leucine; ROS generation was measured by oxidation of dichlorodihydrofluorescein. The dynamics of ACE activity and intensity of ROS generation in the aorta after catechin administration and their dependence on...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Peptide KE in Human Proteome
Peptide KE exhibits immunoprotective, geroprotective, and oncostatic activities and stimulates functional activity of fibroblasts. The KE motif is present in amino acid sequences of some cytokines and peptide hormones functionally similar to KE peptide. However, the relationship between the presence of KE motif and protein functions on the scale of known human proteome has not yet received sufficient attention. The incidence of bioregulatory peptide KE in proteins of various functional groups constituting...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Effect of Vanadium Complex with Enzymatic Hydrolysate of Soy Protein on Carbohydrate and Lipid Metabolism Disorders in Male Wistar Rats
The effects of vanadium complex with enzymatic hydrolysate of soy protein (V-EHSPI) were studied in male Wistar rats with induced disorders of carbohydrate and lipid metabolism. The content of vanadium (IV) in the studied complex was 15.8 mg/g dry product. High-lipid high-carbohydrate diet was used to induce disorders of lipid and carbohydrate metabolism. Addition of vanadium in three different doses to the diet over 100-day experiment reduced body weight gain and the levels of glucose, insulin,...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Structural Changes in the Lungs and Liver of Mice with Experimental Tuberculosis Treated with Liposome-Encapsulated Dextrazide
Male BALB/с mice were intravenously infected with Mycobacterium tuberculosis H37Rv (0.5 ml of 2-week culture). One month later, treatment with liposome-encapsulated dextrazide (LEDZ, a conjugate of isonicotinic acid hydrazide (INH) and 40 kDa oxidized dextran encapsulated in phosphatidylcholine liposomes), INH, or a combination of LEDZ with INH was started. The doses of LEDZ (liposome suspension) and INH were 0.025 ml/10 g body weight and 5 mg/kg body weight, respectively. All the substances were...
Latest Results for Bulletin of Experimental Biology and Medicine
2d
Combining measures of immune infiltration shows additive effect on survival prediction in high-grade serous ovarian carcinoma
British Journal of Cancer, Published online: 06 April 2020; doi:10.1038/s41416-020-0822-xCombining measures of immune infiltration shows additive effect on survival prediction in high-grade serous ovarian carcinoma
British Journal of Cancer - Issue - nature.com science feeds
4h
Combining measures of immune infiltration shows additive effect on survival prediction in high-grade serous ovarian carcinoma
British Journal of Cancer - Issue - nature.com science feeds
7h
Correction: Chromatin structure, transcriptional activity and DNA repair efficiency affect the outcome of chemotherapy in multiple myeloma
British Journal of Cancer, Published online: 03 April 2020; doi:10.1038/s41416-020-0830-xCorrection: Chromatin structure, transcriptional activity and DNA repair efficiency affect the outcome of chemotherapy in multiple myeloma
British Journal of Cancer - Issue - nature.com science feeds
2d
Correction: Chromatin structure, transcriptional activity and DNA repair efficiency affect the outcome of chemotherapy in multiple myeloma
British Journal of Cancer - Issue - nature.com science feeds
2d
Development of the Japanese version of the Other As Shamer Scale using item response theory
External shame reflects a person’s anxiety that he or she might be rejected by others. The Other as Shamer Scale (OAS) is a scale for assessing external shame. The Japanese version of the OAS was developed, an...
BMC Research Notes - Latest Articles
5h
Full-term low birth weight infants have differentially hypermethylated DNA related to immune system and organ growth: a comparison with full-term normal birth weight infants
Low birth weight (LBW) is a major public health issue as it increases the risk of noncommunicable diseases throughout life. However, the genome-wide DNA methylation patterns of full-term LBW infants (FT-LBWs) ...
BMC Research Notes - Latest Articles
2d
Discovery of Herbal Pairs Containing Gastrodia elata Based on Data Mining and the Delphi Expert Questionnaire and Their Potential Effects on Stroke through Network Pharmacology
Background. Traditional Chinese medicine (TCM) formulae can be regarded as a source of new antistroke drugs. The aim of this study was to discover herbal pairs containing Gastrodia elata (Tianma, TM) from formulae based on data mining and the Delphi expert questionnaire. The proposed approach for discovering new herbal combinations, which included data mining, a clinical investigation, and a network pharmacology analysis, was evaluated in this study. Methods. A database of formulae containing TM...
Hindawi Publishing Corporation
6h
Massive Pulmonary Hemorrhage from Bronchial Varix
Bronchial varix is a rare pulmonary disorder which may lead to life-threatening hemorrhage. Diagnosis is difficult because of the subtle abnormalities on radiographic and bronchoscopic examination. We present a case of massive hemoptysis from a bleeding bronchial varix. In the absence of immediate complex endobronchial therapy in the island of Guam, this case was initially managed with nebulized and intravenous tranexamic acid. This was followed by endobronchial blockade of the bleeding airway with...
Hindawi Publishing Corporation
1d
Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine
The success of peptide-based dendritic cell (DC) cancer vaccines mainly depends on the utilized peptides and selection of an appropriate adjuvant. Herein, we aimed to evoke a broad immune response against multiple epitopes concurrently in the presence of immunoadjuvant. Three synthetic HLA-A0201-restricted peptides were separately linked with HMGB1-derived peptide (SAFFLFCSE, denoted as HB100-108) as immunoadjuvant via double arginine (RR) linker and loaded onto human monocyte-derived DCs. Peptide...
Hindawi Publishing Corporation
1d
A Toxicological Evaluation of Germanium Sesquioxide (Organic Germanium)
A battery of OECD- and GLP-compliant toxicological studies was performed to assess the safety of a highly purified germanium sesquioxide, an organic form of the naturally occurring, nonessential trace element germanium. Germanium dioxide and germanium lactate citrate (inorganic germaniums) have been shown to induce renal toxicity, whereas germanium sesquioxide (an organic germanium) has been shown to have a more favorable safety profile. However, past toxicity studies on germanium sesquioxide compounds...
Hindawi Publishing Corporation
1d
Combining a Cognitive Concurrent Task with a Motor or Motor-Cognitive Task: Which Is Better to Differentiate Levels of Affectation in Parkinson’s Disease?
Introduction. Cognitive decline usually coexists with motor impairment in PD. Multitask settings provide appropriate measures to evaluate the complex interaction between motor and cognitive impairments. The main objective was to analyze which concurrent task, i. e., motor or hybrid motor-cognitive, in combination with a cognitive task better differentiates between PD patients with mild and moderate levels of disease. Methods. Thirty-seven individuals (19 male and 18 female) with idiopathic PD performed...
Hindawi Publishing Corporation
1d
IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer
Ferroptosis, implicated in several diseases, is a new form of programmed and nonapoptotic cell death triggered by iron-dependent lipid peroxidation after inactivation of the cystine/glutamate antiporter system xc–, which is composed of solute carrier family 7 membrane 11 (SLC7A11) and solute carrier family 3 membrane 2 (SLC3A2). Therefore, inducing ferroptosis through inhibiting the cystine/glutamate antiporter system xc– may be an effective way to treat cancer. In previous screening tests, we found...
Hindawi Publishing Corporation
1d
Lymph Node Metastases of Medullary Thyroid Cancer: Role of Calcitonin in the Washout Fluid of Fine-Needle Aspiration
Introduction. The diagnostic value of calcitonin (CT) measurement in fine-needle aspirate washout (FNA-CT) for medullary thyroid cancer (MTC) lymph node (LN) metastases remains to be determined. It may increase the diagnostic sensitivity, but data on this subject is sparse. Objective. Our study aimed to evaluate the utility of FNA-CT in the diagnosis of LN metastases of MTC. Methods. We retrospectively investigated, in our institutional database, 69 consecutive FNA LN cytology from 42 patients who...
Hindawi Publishing Corporation
1d
Recognition of Point Sets Objects in Realistic Scenes
With the emergence of new intelligent sensing technologies such as 3D scanners and stereo vision, high-quality point clouds have become very convenient and lower cost. The research of 3D object recognition based on point clouds has also received widespread attention. Point clouds are an important type of geometric data structure. Because of its irregular format, many researchers convert this data into regular three-dimensional voxel grids or image collections. However, this can lead to unnecessary...
Hindawi Publishing Corporation
1d
Tensile Behavior of Acrylonitrile Butadiene Styrene at Different Temperatures
Temperature greatly influences the mechanical response of acrylonitrile butadiene styrene (ABS). The tensile behavior of ABS was explored in this study. The tensile experiments were conducted at a wide range of temperatures (from 40°C to 130°C). A model was established to reveal the quantitative relationship between temperature and tensile behavior of ABS. The results of tensile experiments showed that tensile behavior of ABS exhibited glassy state and high-elastics state. The model was also divided...
Hindawi Publishing Corporation
1d

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