The quinoprotein glycine oxidase from the marine bacterium Pseudoalteromonas luteoviolacea (PlGoxA) uses a protein-derived cysteine tryptophylquinone (CTQ) cofactor to catalyze conversion of glycine to glyoxylate and ammonia. This homotetremeric enzyme exhibits strong cooperativity towards glycine binding. It is a good model for studying enzyme kinetics and cooperativity, specifically for being able to separate those aspects of protein function through directed mutagenesis. Variant proteins were...
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tRNAs universally carry a CCA nucleotide triplet at their 3′-ends. In eukaryotes, the CCA is added post-transcriptionally by the CCA-adding enzyme (CAE). The mitochondrion of the parasitic protozoan Trypanosoma brucei lacks tRNA genes and therefore imports all of its tRNAs from the cytosol. This has generated interest in the tRNA modifications and their distribution in this organism, including how CCA is added to tRNAs. Here, using a BLAST search for genes encoding putative CAE proteins in T. brucei,...
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Conopeptides are neurotoxic peptides in the venom of marine cone snails and have broad therapeutic potential for managing pain and other conditions. Here, we identified the single-disulfide peptides Czon1107 and Cca1669 from the venoms of Conus zonatus and Conus caracteristicus, respectively. We observed that Czon1107 strongly inhibits the human α3β4 (IC50 15.7 ± 3.0 μM) and α7 (IC50 77.1 ± 0.05 μM) nicotinic acetylcholine receptor (nAChR) subtypes, but the activity of Cca1669 remains to be identified....
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Tumor cells can spread to distant sites through their ability to switch between mesenchymal and amoeboid (bleb-based) migration. Because of this difference, inhibitors of metastasis must account for each migration mode. However, the role of Vimentin in amoeboid migration has not been determined. Since amoeboid, Leader Bleb-Based Migration (LBBM) occurs in confined spaces and Vimentin is known to strongly influence cell mechanical properties, we hypothesized that a flexible Vimentin network is required...
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Combination of gemcitabine and nab-paclitaxel has superior clinical efficacy than gemcitabine alone. Nevertheless, health-related quality of life. (QoL) associated with this combination therapy when administer...
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Colon cancer is one of the most common malignancies and the fourth leading cause of cancer-related mortality in the world. Colibactin, which is synthesized by the pks genomic island of E. coli interfere with the ...
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Metastatic pancreatic cancer has a median overall survival of less than 12 months, even if treated with chemotherapy. Selected patients with oligometastatic disease could benefit from multimodal treatments con...
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S100 calcium binding protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has...
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Li-Fraumeni syndrome is a cancer predisposition syndrome caused by germline TP53 tumor suppressor gene mutations, with no previous association with pancreatic neuroendocrine tumors (PNETs). Here we present the fi...
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Despite the fact that osteosarcoma is one of the most common primary bone malignancies with poor prognosis, the mechanism behind the pathogenesis of osteosarcoma is only partially known. Here we characterized differentially expressed genes (DEG) by extensive analysis of several publicly available gene expression profile datasets and identified MAFB as a key transcriptional regulator in osteosarcoma progression. MAFB was highly expressed in tumor tissues and required for proliferation and tumorigenicity...
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British Journal of Cancer, Published online: 01 April 2020; doi:10.1038/s41416-020-0796-8Molecular subtypes of oropharyngeal cancer show distinct immune microenvironment related with immune checkpoint blockade response
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Abstract The ongoing pandemic of SARS‐CoV‐2 virus and its associated disease COVID‐19 has resulted in widespread ventilator shortages and rationing of care. Massive global supply chain disruption and quarantine measures prevent equipment movement and medical device production.
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Summary Estimates of the rate and risk‐factors for difficult airway rarely include a denominator for the number of anaesthetics. Approaches such as self‐reporting and crowd‐sourcing of airway incidents may help identify specific lessons from clinical episodes, but the lack of denominator data, biased reporting and under‐reporting does not allow a comprehensive population‐based assessment. We used an established state‐wide dataset to determine the incidence of failed and difficult intubations between...
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Abstract Purpose An increasing body of evidence suggests that dipeptidyl-peptidase 4 (DPP-4) inhibitors could play a role in the development of bullous pemphigoid. The knowledge regarding this association is based on case reports, pharmacovigilance database analyses, and observational studies. Data from randomized clinical trials are a relevant source of information on adverse events. Since no single trial has a sufficient power to...
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Abstract Purpose This study aimed to compare the nigella sativa vs. placebo effect on anthropometric and biochemical indices in postmenopausal women with metabolic syndrome. Methods This randomized, double-blinded, placebo-controlled trial was conducted as a third-phase trial among 140 menopausal women within the age of 45–60 years old, who were suffering...
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Abstract Purpose The diagnosis of primary hyperparathyroidism (PHPT) and chronic hypoparathyroidism (HypoPT) is still challenging, especially in patients asymptomatic or with non-classical phenotypes and for physicians not skilled in calcium-phosphorous (Ca–P) disorders. The serum calcium/phosphorous (Ca/P) ratio has been proposed as accurate index to identify PHPT, while it has never been tested in HypoPT. The aim of this study is...
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The cover image is based on the Original Article Alteration of calcium signalling in cardiomyocyte induced by simulated microgravity and hypergravity by Caizhi Liu et al., https://doi.org/10.1111/cpr.12783.
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Abstract Objectives Spindle and kinetochore–associated protein 1(SKA1), originally identified as a protein essential for proper chromosome segregation, has been recently linked to multiple malignancies. This study aimed to explore the biological, clinical role and molecular mechanism of SKA1 in pancreatic carcinogenesis. Materials and Methods SKA1 expression was detected in 145 pancreatic ductal adenocarcinoma (PDAC) specimens by immunohistochemistry. Biological behaviour assays...
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ACS NanoDOI: 10.1021/acsnano.0c01908
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ACS NanoDOI: 10.1021/acsnano.9b09901
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ACS NanoDOI: 10.1021/acsnano.0c00216
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ACS NanoDOI: 10.1021/acsnano.0c00058
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Osteosarcoma is a malignant bone tumor. Increasing evidences have revealed that a disintegrin and metalloproteinase 10 (ADAM10) is implicated in tumor development. The main purpose of this study is to explore ...
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Colorectal cancer (CRC) is a malignant tumor, and the overall prognosis of patients with advanced CRC is still unsatisfactory. Circular RNAs (circRNAs) vesicle-associated membrane protein-associated protein A ...
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Tumor progression and distant metastasis are the main causes of deaths in colorectal cancer (CRC) patients, and the molecular mechanisms in CRC metastasis have not been completely discovered.
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Osteosarcoma (OS) is one of the most common types of primary bone tumors which poses negative effects on the bones of both young children and adolescents. LncRNA LINC00472 has been reported to be involved with...
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Pancreatic ductal adenocarcinoma (PDAC) is a lethal human malignancy, and previous researches support the contribution of microRNA (miRNA) to cancer progression. MiR-122-5p is reported to participate in the re...
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3D Printing and Additive Manufacturing, Ahead of Print.
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Our study demonstrated that using extensive lymphadenectomy (≥ 5 lymph nodes) would provide a significant survival benefit. The substantial effort is recommended to made to increase the use of lymphadenectomy, radical resection/radical cholecystectomy, and extended surgical resection in the treatment of gall bladder cancer. However, the survival benefit of extended surgical resection was not significantly better than that of simple cholecystectomy, and could have been affected by few patients who...
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The paper identifies patient and center characteristics that account for the use of psycho‐oncological services by prostate cancer patients in certified prostate cancer centers in Germany. Multilevel analysis was conducted. The results indicate that the varying utilization of psycho‐oncological services in certified prostate cancer centers may be better explained by characteristics of the centers and the patients’ clinical characteristics, rather than by the patients’ sociodemographic features. Abstract...
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ADT is standard therapy for advanced and metastatic PC with negative metabolic consequences. We determined the metabolomic effects of ADT during the PC treatments. Such analysis identify the unexpected reduced ketogenesis and microbiome‐associated metabolites during ADT Abstract Androgen deprivation therapy (ADT) is the main treatment strategy for men with metastatic prostate cancer (PC). However, ADT is associated with various metabolic disturbances, including impaired glucose tolerance, insulin...
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Considering the results of past research and our current study, we find that the expression of two major proteins of Peroxiredoxin‐2 and Alpha‐1 antitrypsin is dramatically decreased in the cancerous tissues, and the expression level of Haptoglobin in the cancerous tissue is strongly increased. Abstract Background The development of lung cancer is a multifactorial process that involves the environmental and genetic factors. The mortality rate of this cancer is higher than breast, colorectal,...
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Abstract Pemetrexed (PEM) is a useful drug that can be combined with immune checkpoint blockade therapy for treatment of patients with advanced non‐small‐cell lung cancer (NSCLC). However, its effects on anti‐cancer immunity, especially the sensitivity of NSCLC cells to cytotoxic immune cells, have not been fully investigated. In this study, we examined the effects of PEM on the sensitivity of human NSCLC cells to two different types of cytotoxic immune cells. Pre‐treatment with PEM increased the...
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